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  Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity

Lampert, T. J., Kamprad, N., Edwards, M., Borleis, J., Watson, A. J., Tarantola, M., et al. (2017). Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity. Proceedings of the National Academy of Sciences of the United States of America, 114(37), E7727-E7736. doi:10.1073/pnas.1616600114.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F0EC-F Version Permalink: http://hdl.handle.net/21.11116/0000-0001-74DB-6
Genre: Journal Article

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 Creators:
Lampert, T. J., Author
Kamprad, Nadine1, Author              
Edwards, M., Author
Borleis, J., Author
Watson, A. J., Author
Tarantola, Marco1, Author              
Devreotes, P. N., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              

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Free keywords: cell adhesion; cell migration; chemotaxis; Dictyostelium; PTEN
 Abstract: The model organism Dictyostelium discoideum has greatly facilitated our understanding of the signal transduction and cytoskeletal pathways that govern cell motility. Cell-substrate adhesion is downstream of many migratory and chemotaxis signaling events. Dictyostelium cells lacking the tumor suppressor PTEN show strongly impaired migratory activity and adhere strongly to their substrates. We reasoned that other regulators of migration could be obtained through a screen for overly adhesive mutants. A screen of restriction enzyme-mediated integration mutagenized cells yielded numerous mutants with the desired phenotypes, and the insertion sites in 18 of the strains were mapped. These regulators of adhesion and motility mutants have increased adhesion and decreased motility. Characterization of seven strains demonstrated decreased directed migration, flatness, increased filamentous actin-based protrusions, and increased signal transduction network activity. Many of the genes share homology to human genes and demonstrate the diverse array of cellular networks that function in adhesion and migration.

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Language(s): eng - English
 Dates: 2017-08-28
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1073/pnas.1616600114
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 114 (37) Sequence Number: - Start / End Page: E7727 - E7736 Identifier: -