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  Selective depletion of the acetylcholine and vasoactive intestinal polypeptide of the guinea-pig myenteric plexus by differential mobilization of distinct transmitter pools.

Agoston, D., Conlon, J. M., & Whittaker, V. P. (1988). Selective depletion of the acetylcholine and vasoactive intestinal polypeptide of the guinea-pig myenteric plexus by differential mobilization of distinct transmitter pools. Experimental Brain Research, 72(3), 535-642. doi:10.1007/BF00250599.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F34E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F350-F
Genre: Journal Article

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Agoston, D.1, Author              
Conlon, J. M., Author
Whittaker, V. P.1, Author              
Affiliations:
1Abteilung Neurochemie, MPI for biophysical chemistry, Max Planck Society, ou_578555              

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 Abstract: The effect of electrical field stimulation on the release of acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) from superfused strips of myenteric plexus-longitudinal muscle (MPLM) of guinea-pig ileum and on the transmitter content of the tissue was investigated at different frequencies and in the presence and absence of choline hemicholinium-3 and colchicine. Low frequency electrical field stimulation released ACh by more than 4 times the basal release; the simultaneously detected VIP secretion was increased only slightly above the resting level. During high frequency stimulation (50 Hz) the release of VIP was greatly increased (to 5 times the resting release) whereas the release of ACh increased to only 150% of the basal output. When choline was present, the ACh content of the tissue itself was not altered by electrical stimulation indicating a rate of synthesis sufficient to maintain release. It was reduced in a frequency-dependent manner in the absence of exogenous choline or in the presence of 10 microM hemicholinium-3 (an inhibitor of choline uptake) by up to 54% of the original content. A similar but even larger reduction took place in the amount of ACh released. Neither the secretion of VIP nor the tissue VIP content was altered by these treatments. Long-lasting (greater than 60 min) high-frequency (50 Hz) stimulation resulted in the depletion of the VIP pool (by 25%) while the ACh content remained unaltered.

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Language(s): eng - English
 Dates: 1988
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/BF00250599
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Title: Experimental Brain Research
Source Genre: Journal
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Pages: - Volume / Issue: 72 (3) Sequence Number: - Start / End Page: 535 - 642 Identifier: -