English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Optical recording of electrical activity from axons and glia of frog optic nerve: Potentiometric dye responses and morphometrics.

Konnerth, A., Orkand, P. M., & Orkand, R. K. (1988). Optical recording of electrical activity from axons and glia of frog optic nerve: Potentiometric dye responses and morphometrics. Glia, 1(3), 225-232. doi:10.1002/glia.440010308.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F35E-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F360-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Konnerth, A.1, Author              
Orkand, P. M., Author
Orkand, R. K., Author
Affiliations:
1Research Group of Cellular Neurophysiology, MPI for biophysical chemistry, Max Planck Society, ou_578557              

Content

show
hide
Free keywords: -
 Abstract: Voltage-sensitive dyes were used to study the changes in membrane potential in axons and glial cells of the frog optic nerve following electrical stimulation. The lack of a signal in the unstained nerve and the multiphasic action spectra after staining indicated that the optical responses were from the extrinsic dyes. Changes in dye absorption and fluorescence had rapid and slow phases. The rapid phases resulted from action potentials in myelinated and unmyelinated axons. The kinetics of the slow phase of the optical response were similar to the depolarization recorded from the glial cells with intracellular electrodes. The ratio of the amplitudes of the fast and slow phases was characteristic for each type of dye. Pharmacological analysis of the action potential of the unmyelinated axons revealed tetrodotoxin-sensitive sodium channels and 4-aminopyridine-sensitive potassium channels. Repeated exposure of the stained preparation to light led to photodynamic damage as shown by a block of recovery of the glial depolarization. An electron microscopic morphometric study of the nerve was carried out in an effort to understand the contribution of the various anatomical elements to the compound optical response. The ratio of unmyelinated axon membrane to glial membrane was much greater than was the ratio of the fast and slow components of the signal, suggesting that the dyes either had a higher affinity for glial membrane or did not penetrate the nerve uniformly.

Details

show
hide
Language(s): eng - English
 Dates: 1988
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/glia.440010308
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Glia
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1 (3) Sequence Number: - Start / End Page: 225 - 232 Identifier: -