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  Cloning and sequence analysis of a cDNA from seminal vesicle tissue encoding the precursor of the major protein of bull semen.

Kemme, M., & Scheit, K. (1988). Cloning and sequence analysis of a cDNA from seminal vesicle tissue encoding the precursor of the major protein of bull semen. DNA, 7(9), 595-599. doi:10.1089/dna.1988.7.595.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F57E-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-F581-2
Genre: Journal Article

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 Creators:
Kemme, M.1, Author              
Scheit, K.H.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with a synthetic DNA probe specific for the major protein of bull semen. A positive clone pMP17, containing a 680-bp insert, was sequenced. In combination with primer-extension sequencing of poly(A)+RNA, a DNA sequence of 700 bp was determined. This DNA encodes a reading frame for 134 amino acids, starting with an ATG and terminated by a TAG codon. The first 25 amino acids constitute a signal peptide segment followed by 109 amino acids with the known sequence of the major protein. The initiation methionine occurs within the sequence CTACCATGG, which is highly homologous to a putative control signal for translational efficiency of mammalian mRNAs. The DNA sequence comprises a 3' untranslated region of 276 bp and the polyadenylation signal AATAAA, 13 bp upstream from a tract of A residues. Northern analysis indicated the presence of a 750-bp mRNA species in poly(A)+RNA of seminal vesicle tissue. According to Southern analysis, one gene appears to specify the major protein of bull semen.

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Language(s): eng - English
 Dates: 1988-11
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1089/dna.1988.7.595
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Title: DNA
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 7 (9) Sequence Number: - Start / End Page: 595 - 599 Identifier: -