English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  In vivo isotopic labeling of symbiotic bacteria involved in cellulose degradation and nitrogen recycling within the gut of the forest cockchafer (Melolontha hippocastani)

Alonso, P., Bartram, S., Arias-Cordero, E. M., Novoselov, A., Halty-deLeon, L., Shao, Y., et al. (2017). In vivo isotopic labeling of symbiotic bacteria involved in cellulose degradation and nitrogen recycling within the gut of the forest cockchafer (Melolontha hippocastani). Frontiers in Microbiology, 8: 1970. doi:10.3389/fmicb.2017.01970.

Item is

Files

show Files
hide Files
:
BOL685.pdf (Publisher version), 2MB
Name:
BOL685.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
BOL685s1.docx (Supplementary material), 14MB
Name:
BOL685s1.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
:
BOL685e.pdf (Publisher version), 71KB
Name:
BOL685e.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.3389/fmicb.2017.01970 (Publisher version)
Description:
OA

Creators

show
hide
 Creators:
Alonso, Pol1, Author              
Bartram, Stefan1, Author              
Arias-Cordero, Erika M.1, 2, Author              
Novoselov, Alexey1, 2, Author              
Halty-deLeon, Lorena, Author
Shao, Yongqi, Author
Boland, Wilhelm1, Author              
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

Content

show
hide
Free keywords: -
 Abstract: The guts of insects harbor symbiotic bacterial communities. However, due to their complexity, it is challenging to relate a specific symbiotic phylotype to its corresponding function. In the present study, we focused on the forest cockchafer (Melolontha hippocastani), a phytophagous insect with a dual life cycle, consisting of a root-feeding larval stage and a leaf-feeding adult stage. By combining in vivo stable isotope probing with 13C cellulose and 15N urea as trophic links, with Illumina MiSeq (Illumina-SIP), we unraveled bacterial networks processing recalcitrant dietary components and recycling nitrogenous waste. The bacterial communities behind these processes change between larval and adult stages. In 13C cellulose-fed insects, the bacterial families Lachnospiraceae and Enterobacteriaceae were isotopically labeled in larvae and adults, respectively. In 15N urea-fed insects, the genera Burkholderia and Parabacteroides were isotopically labeled in larvae and adults, respectively. Additionally, the PICRUSt-predicted metagenome suggested a possible ability to degrade hemicellulose and to produce amino acids of, respectively, 13C cellulose- and 15N urea labeled bacteria. The incorporation of 15N from ingested urea back into the insect body was confirmed, in larvae and adults, by isotope ratio mass spectrometry (IRMS). Besides highlighting key bacterial symbionts of the gut of M. hippocastani, this study provides example on how Illumina-SIP with multiple trophic links can be used to target microorganisms embracing different roles within an environment.

Details

show
hide
Language(s):
 Dates: 2017-09-252017-10-122017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: BOL685
DOI: 10.3389/fmicb.2017.01970
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in Microbiology
  Abbreviation : Front. Microbiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 8 Sequence Number: 1970 Start / End Page: - Identifier: ISSN: 1664-302X
CoNE: https://pure.mpg.de/cone/journals/resource/1664-302X