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  Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.

Kliesch, T. T., Dietz, J., Turco, L., Halder, P., Polo, E., Tarantola, M., et al. (2017). Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs. Scientific Reports, 7: 12070. doi:10.1038/s41598-017-12348-w.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-FCE5-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-FCE9-4
Genre: Journal Article

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 Creators:
Kliesch, T. T., Author
Dietz, J., Author
Turco, L.1, Author              
Halder, P.2, Author              
Polo, E., Author
Tarantola, M.1, Author              
Jahn, R.2, Author              
Janshoff, A., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              
2Department of Neurobiology, MPI for Biophysical Chemistry, Max Planck Society, ou_578595              

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 Abstract: The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m−1, at which the number of fusion events is increased substantially.

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Language(s): eng - English
 Dates: 2017-09-21
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41598-017-12348-w
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Title: Scientific Reports
Source Genre: Journal
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Pages: 13 Volume / Issue: 7 Sequence Number: 12070 Start / End Page: - Identifier: -