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  Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom

Xing, P., Hahnke, R. L., Unfried, F., Markert, S., Huang, S., Barbeyron, T., et al. (2015). Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. The ISME Journal, 9: 1, pp. 1410-1422.

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 Creators:
Xing, Peng1, Author           
Hahnke, Richard L.2, Author           
Unfried, Frank3, Author           
Markert, Stephanie, Author
Huang, Sixing3, Author           
Barbeyron, Tristan, Author
Harder, Jens2, Author           
Becher, Dörte, Author
Schweder, Thomas, Author
Glöckner, Frank Oliver1, Author           
Amann, Rudolf I.3, Author           
Teeling, Hanno3, Author           
Affiliations:
1Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481697              
2Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481695              
3Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: Members of the flavobacterial genus Polaribacter thrive in response to North Sea spring phytoplankton blooms. We analyzed two respective Polaribacter species by whole genome sequencing, comparative genomics, substrate tests and proteomics. Both can degrade algal polysaccharides but occupy distinct niches. The liquid culture isolate Polaribacter sp. strain Hel1_33_49 has a 3.0-Mbp genome with an overall peptidase:CAZyme ratio of 1.37, four putative polysaccharide utilization loci (PULs) and features proteorhodopsin, whereas the agar plate isolate Polaribacter sp. strain Hel1_85 has a 3.9-Mbp genome with an even peptidase:CAZyme ratio, eight PULs, a mannitol dehydrogenase for decomposing algal mannitol-capped polysaccharides but no proteorhodopsin. Unlike other sequenced Polaribacter species, both isolates have larger sulfatase-rich PULs, supporting earlier assumptions that Polaribacter take part in the decomposition of sulfated polysaccharides. Both strains grow on algal laminarin and the sulfated polysaccharide chondroitin sulfate. For strain Hel1_33_49, we identified by proteomics (i) a laminarin-induced PUL, (ii) chondroitin sulfate-induced CAZymes and (iii) a chondroitin-induced operon that likely enables chondroitin sulfate recognition. These and other data suggest that strain Hel1_33_49 is a planktonic flavobacterium feeding on proteins and a small subset of algal polysaccharides, while the more versatile strain Hel1_85 can decompose a broader spectrum of polysaccharides and likely associates with algae.

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Language(s): eng - English
 Dates: 2014-12-052015-01-01
 Publication Status: Issued
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Internal
 Identifiers: eDoc: 713108
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Title: The ISME Journal
  Other : The ISME journal : multidisciplinary journal of microbial ecology
Source Genre: Journal
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Publ. Info: Basingstoke : Nature Publishing Group
Pages: - Volume / Issue: 9 Sequence Number: 1 Start / End Page: 1410 - 1422 Identifier: ISSN: 1751-7370
CoNE: https://pure.mpg.de/cone/journals/resource/1751-7370