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  Symbiont-host relationships in chemosynthetic mussels: A comprehensive lipid biomarker study

Kellermann, M. Y., Schubotz, F., Elvert, M., Lipp, J. S., Birgel, D., Prieto-Mollar, X., et al. (2012). Symbiont-host relationships in chemosynthetic mussels: A comprehensive lipid biomarker study. Organic Geochemistry, 43, 112-124.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-C887-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-634B-6
Genre: Journal Article

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 Creators:
Kellermann, M. Y., Author
Schubotz, F., Author
Elvert, M.1, Author              
Lipp, J. S., Author
Birgel, D., Author
Prieto-Mollar, X., Author
Dubilier, N.2, Author              
Hinrichs, K. U., Author
Affiliations:
1Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              
2Department of Symbiosis, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481699              

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 Abstract: Symbiosis with chemosynthetic microorganisms allows invertebrates from hydrothermal vents and cold seeps, such as mussels, snails and tubeworms, to gain nutrition independently of organic input from photosynthetic communities. Lipid biomarkers and their compound specific stable carbon isotopes (δ13C) have greatly aided the elucidation of chemosynthetic symbiosis. Due to recent methodological advances in liquid chromatography it is now possible to obtain a more holistic view of lipid biomarkers, including the analysis of intact polar membrane lipids (IPLs) and bacteriohopanepolyols (BHPs). This study provides an extensive examination of polar and apolar lipids in combination with stable carbon isotope analysis of three Bathymodiolus mussels (Bathymodiolus childressi, Bathymodiolus cf. thermophilus, Bathymodiolus brooksi) hosting different types of bacterial symbiont (methane-oxidizing, sulfur-oxidizing and a dual symbiosis with methane- and sulfur-oxidizing symbionts, respectively). We propose that IPLs with C16:1 acyl side chains, and phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) head groups, which were only detected in the gill tissue, can be used as symbiont-characteristic biomarkers. These putative symbiont-specific IPLs provide the opportunity to detect and quantify the methanotrophic and thiotrophic symbionts within the gill tissue. Additional characteristic markers for methanotrophic symbionts were found in B. childressi and B. brooksi, including the BHP derivatives aminotriol and aminotetrol, 4-methyl sterols and diagnostic fatty acids (FAs), such as C16:1ω9, C16:1ω8, and C18:1ω8. In general, the δ13C values of FAs, alcohols and BHP-derived hopanols were in accordance with carbon assimilation pathways of the respective methanotrophic or thiotrophic symbionts in all three Bathymodiolus mussels. Differences in BHP distribution as well as δ13C values in the two mussels hosting a methanotrophic symbiont may indicate the presence of different methanotrophic symbionts and/or changes in the nutritional status. In all three mussel species the δ13C values of lipid biomarkers assigned to the symbionts were similar to those of the hosts, indicating the importance of the bacterial symbionts as the main carbon source for the mussel tissue.

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Language(s): eng - English
 Dates: 2012-02
 Publication Status: Published in print
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 634730
ISI: 000299815000012
 Degree: -

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Title: Organic Geochemistry
Source Genre: Journal
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Publ. Info: Oxford : Pergamon
Pages: - Volume / Issue: 43 Sequence Number: - Start / End Page: 112 - 124 Identifier: ISSN: 0146-6380
CoNE: https://pure.mpg.de/cone/journals/resource/954925474428