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  Benzoate Mediates Repression of C(4)-Dicarboxylate Utilization in "Aromatoleum aromaticum" EbN1

Trautwein, K., Grundmann, O., Woehlbrand, L., Eberlein, C., Boll, M., & Rabus, R. (2012). Benzoate Mediates Repression of C(4)-Dicarboxylate Utilization in "Aromatoleum aromaticum" EbN1. Journal of Bacteriology, 194(2), 518-528.

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 Creators:
Trautwein, K.1, Author              
Grundmann, O.2, Author              
Woehlbrand, L.1, Author              
Eberlein, C., Author
Boll, M., Author
Rabus, R.1, Author              
Affiliations:
1Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481695              
2Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481692              

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 Abstract: Diauxic growth was observed in anaerobic C4-dicarboxylate-adapted cells of “Aromatoleum aromaticum” EbN1 due to preferred benzoate utilization from a substrate mixture of a C4-dicarboxylate (succinate, fumarate, or malate) and benzoate. Differential protein profiles (two-dimensional difference gel electrophoresis [2D DIGE]) revealed dynamic changes in abundance for proteins involved in anaerobic benzoate catabolism and C4-dicarboxylate uptake. In the first active growth phase, benzoate utilization was paralleled by maximal abundance of proteins involved in anaerobic benzoate degradation (e.g., benzoyl-coenzyme A [CoA] reductase) and minimal abundance of DctP (EbA4158), the periplasmic binding protein of a predicted C4-dicarboxylate tripartite ATP-independent periplasmic (TRAP) transporter (DctPQM). The opposite was observed during subsequent succinate utilization in the second active growth phase. The increased dctP (respectively, dctPQM) transcript and DctP protein abundance following benzoate depletion suggests that repression of C4-dicarboxylate uptake seems to be a main determinant for the observed diauxie.

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Language(s): eng - English
 Dates: 2012-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 634736
ISI: 000298677400033
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Title: Journal of Bacteriology
  Other : J. Bacteriol.
Source Genre: Journal
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Publ. Info: Washington, DC : American Society for Microbiology (ASM)
Pages: - Volume / Issue: 194 (2) Sequence Number: - Start / End Page: 518 - 528 Identifier: ISSN: 0021-9193
CoNE: https://pure.mpg.de/cone/journals/resource/954925410823