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  Analysis of 23S rRNA genes in metagenomes - A case study from the Global Ocean Sampling Expedition

Yilmaz, P., Kottmann, R., Pruesse, E., Quast, C., & Gloeckner, F. O. (2011). Analysis of 23S rRNA genes in metagenomes - A case study from the Global Ocean Sampling Expedition. Systematic and Applied Microbiology, 34(6), 462-469.

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Yilmaz, P.1, Author              
Kottmann, R.1, Author              
Pruesse, E.1, Author              
Quast, C.1, Author              
Gloeckner, F. O.1, Author              
Affiliations:
1Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481697              

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 Abstract: As an evolutionary marker, 23S ribosomal RNA (rRNA) offers more diagnostic sequence stretches and greater sequence variation than 16S rRNA. However, 23S rRNA is still not as widely used. Based on 80 metagenome samples from the Global Ocean Sampling (GOS) Expedition, the usefulness and taxonomic resolution of 23S rRNA were compared to those of 16S rRNA. Since 23S rRNA is approximately twice as large as 16S rRNA, twice as many 23S rRNA gene fragments were retrieved from the GOS reads than 16S rRNA gene fragments, with 23S rRNA gene fragments being generally about 100 bp longer. Datasets for 16S and 23S rRNA sequences revealed similar relative abundances for major marine bacterial and archaeal taxa. However, 16S rRNA sequences had a better taxonomic resolution due to their significantly larger reference database. Reevaluation of the specificity of previously published PCR amplification primers and group specific fluorescence in situ hybridization probes on this metagenomic set of non-amplified 23S rRNA sequences revealed that out of 16 primers investigated, only two had more than 90% target group coverage. Evaluations of two probes, BET42a and GAM42a, were in accordance with previous evaluations, with a discrepancy in the target group coverage of the GAM42a probe when evaluated against the GOS metagenomic dataset.

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Language(s): eng - English
 Dates: 2011-09
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 596146
ISI: 000295743800012
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Title: Systematic and Applied Microbiology
  Other : System. Appl. Microbiol.
Source Genre: Journal
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Publ. Info: Stuttgart : Urban & Fischer
Pages: - Volume / Issue: 34 (6) Sequence Number: - Start / End Page: 462 - 469 Identifier: ISSN: 0723-2020
CoNE: https://pure.mpg.de/cone/journals/resource/954928582871