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  Microbial conversion of inorganic carbon to dimethyl sulfide in anoxic lake sediment (Plusssee, Germany)

Lin, Y. S., Heuer, V. B., Ferdelman, T. G., & Hinrichs, K. U. (2010). Microbial conversion of inorganic carbon to dimethyl sulfide in anoxic lake sediment (Plusssee, Germany). Biogeosciences, 7(8), 2433-2444.

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Lin, Y. S., Author
Heuer, V. B., Author
Ferdelman, T. G.1, Author           
Hinrichs, K. U., Author
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1Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

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 Abstract: In anoxic environments, volatile methylated sulfides like methanethiol (MT) and dimethyl sulfide (DMS) link the pools of inorganic and organic carbon with the sulfur cycle. However, direct formation of methylated sulfides from reduction of dissolved inorganic carbon has previously not been demonstrated. When studying the effect of temperature on hydrogenotrophic microbial activity, we observed formation of DMS in anoxic sediment of Lake Plußsee at 55 °C. Subsequent experiments strongly suggested that the formation of DMS involves fixation of bicarbonate via a reductive pathway in analogy to methanogenesis and engages methylation of MT. DMS formation was enhanced by addition of bicarbonate and further increased when both bicarbonate and H2 were supplemented. Inhibition of DMS formation by 2-bromoethanesulfonate points to the involvement of methanogens. Compared to the accumulation of DMS, MT showed the opposite trend but there was no apparent 1:1 stoichiometric ratio between both compounds. Both DMS and MT had negative δ13C values of −62‰ and −55‰, respectively. Labeling with NaH13CO3 showed more rapid incorporation of bicarbonate into DMS than into MT. The stable carbon isotopic evidence implies that bicarbonate was fixed via a reductive pathway of methanogenesis, and the generated methyl coenzyme M became the methyl donor for MT methylation. Neither DMS nor MT accumulation were stimulated by addition of the methyl-group donors methanol and syringic acid or by the methyl-group acceptor hydrogen sulphide. The source of MT was further investigated in a H235S labeling experiment, which demonstrated a microbially-mediated process of hydrogen sulfide methylation to MT that accounted for only <10% of the accumulation rates of DMS. Therefore, the major source of the 13C-depleted MT was neither bicarbonate nor methoxylated aromatic compounds. Other possibilities for isotopically depleted MT, such as other organic precursors like methionine, are discussed. This DMS-forming pathway may be relevant for anoxic environments such as hydrothermally influenced sediments and fluids and sulfate-methane transition zones in marine sediments.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Issued
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 537452
ISI: 000281431800010
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Title: Biogeosciences
  Other : Biogeosciences
Source Genre: Journal
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Publ. Info: Katlenburg-Lindau, Germany : Copernicus GmbH on behalf of the European Geosciences Union
Pages: - Volume / Issue: 7 (8) Sequence Number: - Start / End Page: 2433 - 2444 Identifier: ISSN: 1726-4170
CoNE: https://pure.mpg.de/cone/journals/resource/111087929276006