English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Direct analysis of sulfate reducing bacterial communities in gas hydrate-impacted marine sediments by PCR-DGGE

Bagwell, C., Formolo, M., Ye, Q., Yeager, C., Lyons, T., & Zhang, C. (2009). Direct analysis of sulfate reducing bacterial communities in gas hydrate-impacted marine sediments by PCR-DGGE. Journal of Basic Microbiology, 49, S87-S92.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Formolo9.pdf (Publisher version), 101KB
 
File Permalink:
-
Name:
Formolo9.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bagwell, C.E., Author
Formolo, M.1, Author              
Ye, Q., Author
Yeager, C.M., Author
Lyons, T.W., Author
Zhang, C.L., Author
Affiliations:
1Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

Content

show
hide
Free keywords: -
 Abstract: Molecular investigations of the sulfate reducing bacteria that target the dissimilatory sulfite- reductase subunit A gene (dsrA) are plagued by the nonspecific performance of conventional PCR primers. Here we describe the incorporation of the FailSafe™ PCR System to optimize en- vironmental analysis of dsrA by PCR amplification and denaturing gradient gel electrophoresis. PCR–DGGE analysis of dsrA composition revealed that SRB diversity was greater and more variable throughout the vertical profile of a marine sediment core obtained from a gas hydrate site (GC234) in the Gulf of Mexico than in a sediment core collected from a nearby site devoid of gas hydrates (NBP). Depth profiled dsrB abundance corresponded with sulfate reduction rates at both sites, though measurements were higher at GC234. This study exemplifies the numeri- cal and functional importance of sulfate reducing bacteria in deep-sea sedimentary environ- ments, and incremental methodological advancements, as described herein, will continue to streamline the analysis of sulfate reducer communities in situ.

Details

show
hide
Language(s): eng - English
 Dates: 2009-08-28
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 463874
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Basic Microbiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Berlin : Akademie-Verlag
Pages: - Volume / Issue: 49 Sequence Number: - Start / End Page: S87 - S92 Identifier: ISSN: 0233-111X
CoNE: https://pure.mpg.de/cone/journals/resource/954927579054