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  Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltical SH1(T)

Hieu, C. X., Voigt, B., Albrecht, D., Becher, D., Lombardot, T., Gloeckner, F. O., et al. (2008). Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltical SH1(T). Proteomics, 8(8), 1608-1623.

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 Urheber:
Hieu, C. X., Autor
Voigt, B., Autor
Albrecht, D., Autor
Becher, D., Autor
Lombardot, T.1, Autor           
Gloeckner, F. O.2, Autor           
Amann, R.1, Autor           
Hecker, M., Autor
Schweder, T., Autor
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              
2Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481697              

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Schlagwörter: master gel; Rhodopirellula baltica
 Zusammenfassung: Rhodopirellula baltica SH1T, which was isolated from the water column of the Kieler Bight, a bay in the southwestern Baltic Sea, is a marine aerobic, heterotrophic representative of the ubiquitous bacterial phylum Planctomycetes. We analyzed the R. baltica proteome by applying different preanalytical protein as well as peptide separation techniques (1‐D and 2‐DE, HPLC separation) prior to MS. That way, we could identify a total of 1115 nonredundant proteins from the intracellular proteome and from different cell wall protein fractions. With the contribution of 709 novel proteins resulting from this study, the current comprehensive R. baltica proteomic dataset consists of 1267 unique proteins (accounting for 17.3% of the total putative protein‐coding ORFs), including 261 proteins with a predicted signal peptide. The identified proteins were functionally categorized using Clusters of Orthologous Groups (COGs), and their potential cellular locations were predicted by bioinformatic tools. A unique protein family that contains several YTV domains and is rich in cysteine and proline was found to be a component of the R. baltica proteinaceous cell wall. Based on this comprehensive proteome analysis a global schema of the major metabolic pathways of growing R. baltica cells was deduced.

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Sprache(n): eng - English
 Datum: 2008-04-11
 Publikationsstatus: Erschienen
 Seiten: 17
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 423591
ISI: 000255314300008
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Titel: Proteomics
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Weinheim : WILEY-VCH
Seiten: - Band / Heft: 8 (8) Artikelnummer: - Start- / Endseite: 1608 - 1623 Identifikator: ISSN: 1615-9853
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000294310