English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Insights into the genome of large sulfur bacteria revealed by analysis of single filaments

Mussmann, M., Hu, F. Z., Richter, M., de Beer, D., Preisler, A., Jørgensen, B. B., et al. (2007). Insights into the genome of large sulfur bacteria revealed by analysis of single filaments. PLoS Biology, 5(9): e230, pp. 1923-1937.

Item is

Files

show Files
hide Files
:
Mussmann7.PDF (Publisher version), 870KB
Name:
Mussmann7.PDF
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Mussmann, M.1, Author           
Hu, F. Z., Author
Richter, M.2, Author           
de Beer, D.3, Author           
Preisler, A.4, Author           
Jørgensen, B. B.4, Author           
Huntemann, M.2, Author           
Glöckner, F. O.2, Author           
Amann, R.1, Author           
Koopman, W. J. H., Author
Lasken, R. S., Author
Janto, B., Author
Hogg, J., Author
Stoodley, P., Author
Boissy, R., Author
Ehrlich, G. D., Author
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              
2Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481697              
3Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              
4Department of Biogeochemistry, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481693              

Content

show
hide
Free keywords: -
 Abstract: Marine sediments are frequently covered by mats of the filamentous Beggiatoa and other large nitrate-storing bacteria that oxidize hydrogen sulfide using either oxygen or nitrate, which they store in intracellular vacuoles. Despite their conspicuous metabolic properties and their biogeochemical importance, little is known about their genetic repertoire because of the lack of pure cultures. Here, we present a unique approach to access the genome of single filaments of Beggiatoa by combining whole genome amplification, pyrosequencing, and optical genome mapping. Sequence assemblies were incomplete and yielded average contig sizes of approximately 1 kb. Pathways for sulfur oxidation, nitrate and oxygen respiration, and CO2 fixation confirm the chemolithoautotrophic physiology of Beggiatoa. In addition, Beggiatoa potentially utilize inorganic sulfur compounds and dimethyl sulfoxide as electron acceptors. We propose a mechanism of vacuolar nitrate accumulation that is linked to proton translocation by vacuolar-type ATPases. Comparative genomics indicates substantial horizontal gene transfer of storage, metabolic, and gliding capabilities between Beggiatoa and cyanobacteria. These capabilities enable Beggiatoa to overcome non-overlapping availabilities of electron donors and acceptors while gliding between oxic and sulfidic zones. The first look into the genome of these filamentous sulfur-oxidizing bacteria substantially deepens the understanding of their evolution and their contribution to sulfur and nitrogen cycling in marine sediments.

Details

show
hide
Language(s): eng - English
 Dates: 2007-08-282007-09
 Publication Status: Issued
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 343263
ISI: 000249552300012
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS Biology
  Other : PLoS Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, California, US : Public Library of Science
Pages: - Volume / Issue: 5 (9) Sequence Number: e230 Start / End Page: 1923 - 1937 Identifier: ISSN: 1544-9173
CoNE: https://pure.mpg.de/cone/journals/resource/111056649444170