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  Magneto Immuno-MR: A novel immunoassay based on biogenic magnetosome nanoparticles

Wacker, R., Ceyhan, B., Alhorn, P., Schüler, D., Lang, C., & Niemeyer, C. M. (2007). Magneto Immuno-MR: A novel immunoassay based on biogenic magnetosome nanoparticles. Biochemical and Biophysical Research Communications, 357(2), 391-396.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-CE4C-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-83FE-7
Genre: Journal Article

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Schueler7.pdf (Publisher version), 365KB
 
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 Creators:
Wacker, R., Author
Ceyhan, B., Author
Alhorn, P., Author
Schüler, D.1, Author              
Lang, C.1, Author              
Niemeyer, C. M., Author
Affiliations:
1Department of Microbiology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481695              

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Free keywords: immuno-PCR; real-time immuno-PCR; real-time detection; biomarker; magnetosome; hepatitis B; automatization; protein analytics
 Abstract: We describe an innovative modification of the Immuno-PCR technology for automatable high sensitive antigen detection. The Magneto Immuno-PCR (M-IPCR) is based on antibody-functionalized biogenic magnetosome nanoparticles revealing major advantages over synthetic magnetic particles. The general principle of the M-IPCR is similar to that of a two-sided (sandwich) immunoassay. However, antibody-functionalized magnetosome conjugates were employed for the immobilization and magnetic enrichment of the signal generating detection complex enabling the establishment of a surface independent immunoassay. To this end, the M-IPCR was carried out by simultaneously tagging the antigen with the reagent for read-out, i.e., a conjugate comprising the specific antibody and DNA fragments, in the presence of the antibody-functionalized magnetosomes. To demonstrate the general functionality of the M-IPCR, the detection of recombinant Hepatitis B surface Antigen (HBsAg) in human serum was established. We observed a detection limit of 320 pg/ml of HBsAg using the M-IPCR, which was about 100-fold more sensitive than the analogous Magneto-ELISA, established in parallel for comparison purposes.

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Language(s): eng - English
 Dates: 2007-06-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 344867
ISI: 000246253700011
 Degree: -

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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 357 (2) Sequence Number: - Start / End Page: 391 - 396 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1