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  Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b

Borriss, M., Lombardot, T., Glöckner, F. O., Becher, D., Albrecht, D., & Schweder, T. (2007). Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b. Extremophiles, 11(1), 95-104.

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Gloeckner7.pdf (Publisher version), 567KB
 
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 Creators:
Borriss, M., Author
Lombardot, T.1, Author              
Glöckner, F. O.2, Author              
Becher, D., Author
Albrecht, D., Author
Schweder, T., Author
Affiliations:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              
2Microbial Genomics Group, Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481697              

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Free keywords: psychrophilic Flavobacterium bacteriophage phi 11b; genomics; proteome; low GC
 Abstract: Virion DNA of bacteriophage 11b (Φ11b), which infects a psychrophilic Flavobacterium isolate from Arctic sea-ice, was determined to consist of 36,012 bp. With 30.6% its GC content corresponds to that of host-genus species and is the lowest of all phages of Gram-negative bacteria sequenced so far. Similarities of several of 65 predicted ORFs, genome organization and phylogeny suggest an affiliation to ‘mesophilic’ nonmarine siphoviruses, e.g. to bacteriophages SPP1 and HK97. Early genes presumably encode an essential recombination factor (ERF), a single strand binding (SSB) protein, an endonuclease, and a DNA methylase. The late gene segment is likely to contain a terminase, portal, minor head, protease and a major capsid gene. Five ORFs exhibited similarities to Bacteroidetes species and seem to reflect the host specificity of the phage. Among PAGE-separated virion proteins that were identified by MALDI-ToF mass spectrometry are the portal, the major capsid, and a putative conserved tail protein. The Φ11b genome is the first to be described of a cultivated virus infecting a psychrophilic host as well as a Bacteroidetes bacterium.

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Language(s): eng - English
 Dates: 2006-08-252007-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 345650
ISI: 000244249100012
 Degree: -

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Title: Extremophiles
  Other : Extremophiles
Source Genre: Journal
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Publ. Info: Berlin : Springer
Pages: - Volume / Issue: 11 (1) Sequence Number: - Start / End Page: 95 - 104 Identifier: ISSN: 1431-0651
CoNE: https://pure.mpg.de/cone/journals/resource/954925623254