日本語
 
Help Privacy Policy ポリシー/免責事項
  詳細検索ブラウズ

アイテム詳細

登録内容を編集ファイル形式で保存
 
 
ダウンロード電子メール
  Diversity and distribution of methanotrophic archaea at cold seeps

Knittel, K., Lösekann, T., Boetius, A., Kort, R., & Amann, R. (2005). Diversity and distribution of methanotrophic archaea at cold seeps. Applied and Environmental Microbiology, 71(1), 467-479.

Item is

基本情報

表示: 非表示:
アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0001-D085-D 版のパーマリンク: https://hdl.handle.net/21.11116/0000-0007-8F92-3
資料種別: 学術論文

ファイル

表示: ファイル
非表示: ファイル
:
Knittel5.pdf (出版社版), 870KB
ファイルのパーマリンク:
https://hdl.handle.net/21.11116/0000-0007-8F93-2
ファイル名:
Knittel5.pdf
説明:
-
OA-Status:
閲覧制限:
公開
MIMEタイプ / チェックサム:
application/pdf / [MD5]
技術的なメタデータ:
著作権日付:
-
著作権情報:
-
CCライセンス:
-

関連URL

表示:

作成者

表示:
非表示:
 作成者:
Knittel, K.1, 著者           
Lösekann, T.1, 著者           
Boetius, A.2, 著者           
Kort, R., 著者
Amann, R.1, 著者           
所属:
1Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              
2HGF MPG Joint Research Group for Deep Sea Ecology & Technology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481702              

内容説明

表示:
非表示:
キーワード: -
 要旨: In this study we investigated by using 16S rRNA-based methods the distribution and biomass of archaea in samples from (i) sediments above outcropping methane hydrate at Hydrate Ridge (Cascadia margin off Oregon) and (ii) massive microbial mats enclosing carbonate reefs (Crimea area, Black Sea). The archaeal diversity was low in both locations; there were only four (Hydrate Ridge) and five (Black Sea) different phylogenetic clusters of sequences, most of which belonged to the methanotrophic archaea (ANME). ANME group 2 (ANME-2) sequences were the most abundant and diverse sequences at Hydrate Ridge, whereas ANME-1 sequences dominated the Black Sea mats. Other seep-specific sequences belonged to the newly defined group ANME-3 (related to Methanococcoides spp.) and to the Crenarchaeota of marine benthic group B. Quantitative analysis of the samples by fluorescence in situ hybridization (FISH) showed that ANME-1 and ANME-2 co-occurred at the cold seep sites investigated. At Hydrate Ridge the surface sediments were dominated by aggregates consisting of ANME-2 and members of the Desulfosarcina-Desulfococcus branch (DSS) (ANME-2/DSS aggregates), which accounted for >90% of the total cell biomass. The numbers of ANME-1 cells increased strongly with depth; these cells accounted 1% of all single cells at the surface and more than 30% of all single cells (5% of the total cells) in 7- to 10-cm sediment horizons that were directly above layers of gas hydrate. In the Black Sea microbial mats ANME-1 accounted for about 50% of all cells. ANME-2/DSS aggregates occurred in microenvironments within the mat but accounted for only 1% of the total cells. FISH probes for the ANME-2a and ANME-2c subclusters were designed based on a comparative 16S rRNA analysis. In Hydrate Ridge sediments ANME-2a/DSS and ANME-2c/DSS aggregates differed significantly in morphology and abundance. The relative abundance values for these subgroups were remarkably different at Beggiatoa sites (80% ANME-2a, 20% ANME-2c) and Calyptogena sites (20% ANME-2a, 80% ANME-2c), indicating that there was preferential selection of the groups in the two habitats. These variations in the distribution, diversity, and morphology of methanotrophic consortia are discussed with respect to the presence of microbial ecotypes, niche formation, and biogeography.

資料詳細

表示:
非表示:
言語: eng - English
 日付: 2005-01
 出版の状態: 出版
 ページ: 13
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): eDoc: 250509
ISI: 000226458800059
 学位: -

関連イベント

表示:

訴訟

表示:

Project information

表示:

出版物 1

表示:
非表示:
出版物名: Applied and Environmental Microbiology
  その他 : Appl. Environ. Microbiol.
種別: 学術雑誌
 著者・編者:
所属:
出版社, 出版地: American Society for Microbiology (ASM)
ページ: - 巻号: 71 (1) 通巻号: - 開始・終了ページ: 467 - 479 識別子(ISBN, ISSN, DOIなど): ISSN: 0099-2240
CoNE: https://pure.mpg.de/cone/journals/resource/954927519600