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  A system for imaging variable chlorophyll fluorescence of aquatic phototrophs

Grünwald, B., & Kühl, M. (2004). A system for imaging variable chlorophyll fluorescence of aquatic phototrophs. Ophelia, 58(2), 79-89.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0001-D113-D Version Permalink: http://hdl.handle.net/21.11116/0000-0007-9001-4
Genre: Journal Article

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Grunwald4.pdf (Publisher version), 5MB
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 Creators:
Grünwald, B.1, Author              
Kühl, M.1, Author              
Affiliations:
1Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              

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Free keywords: photosynthesis; fluorescence imaging; corals; Prochloron; endolithic algae
 Abstract: We describe a modular imaging system for mapping the variable chlorophyll fluorescence of photosynthetic organisms and tissues in the aquatic environment. The system is based on an array of blue LED's (470 nm) for excitation of chlorophyll and a modulated CCD camera equipped with a long-pass filter for detection of chlorophyll fluorescence >665 nm. The exposure of the sample with blue actinic light, non-actinic measuring light, and a flash of saturating light is based on a software-controlled sequence of variable pulse-widths of the LED's. The LED and the camera modulation are controlled via a custom designed timer-unit connected to a PC. We present examples of application for mapping the distribution of photosynthetic activity in coral tissue, communities of endolithic algae within coral skeleton as well as in tissue of didemnid ascidians harboring symbiotic cyanobacteria. It is possible to study spatio-temporal patterns of variable fluorescence (and the derived quantum yield of photosystem II-related electron transport) in heterogeneous aquatic systems at sub-mm spatial resolution.

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Language(s): eng - English
 Dates: 2004-08
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 220894
ISI: 000223721800002
 Degree: -

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Title: Ophelia
  Other : Ophelia
Source Genre: Journal
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Publ. Info: Helsingør, Denmark : Marine Biological Laboratory.
Pages: - Volume / Issue: 58 (2) Sequence Number: - Start / End Page: 79 - 89 Identifier: ISSN: 0078-5326
CoNE: https://pure.mpg.de/cone/journals/resource/110978984073915