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  Picobenthic cyanobacterial populations revealed by 16S rRNA- targeted in situ hybridization

Abed, R. M. M., Schönhuber, W., Amann, R., & Garcia-Pichel, F. (2002). Picobenthic cyanobacterial populations revealed by 16S rRNA- targeted in situ hybridization. Environmental Microbiology, 4(7), 375-382.

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 Creators:
Abed, R. M. M.1, Author           
Schönhuber, W.2, Author           
Amann, R.2, Author           
Garcia-Pichel, F., Author
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1Permanent Research Group Microsensor, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481711              
2Department of Molecular Ecology, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481696              

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 Abstract: We report on the morphological identification of a population of benthic cyanobacteria from microbial mats, known previously only from molecular analyses of field samples, based on the retrieval of environmental 16S rRNA sequences. We used in situ hybridization with horseradish peroxidase-labelled oligonucleotide probes designed to target the 16S rRNA of our unidentified population. Two probes were designed and checked for target binding ability and specificity using membrane hybridization against electroblotted bands from a denaturant gradient gel electrophoresis (DGGE) fingerprint of 16S rDNA gene fragments from the original cyanobacterial community. Under in situ hybridization, these probes bound specifically to extremely small, unicellular, colony-forming cyanobacteria, 0.75-1 mum in diameter, which were embedded in abundant mucilaginous investments. We propose the term picobenthos, by analogy with picoplankton, to describe those unicellular benthic microbes around or less than 1 mum in diameter. Although picoplanktonic cyanobacteria are abundant in ocean and freshwaters, picobenthic (<1 μm) unicellular cyanobacteria are not typically recognized as a major component of microbial mats. The small size and low levels of photopigment autofluorescence from these cells probably rendered them cryptic or indistinguishable from heterotrophic bacteria in routine microscopic observations. It is not known how widespread picobenthic cyanobacteria may be in other environments.

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Language(s): eng - English
 Dates: 2002-07-18
 Publication Status: Issued
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 13863
ISI: 000176906400001
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Title: Environmental Microbiology
  Other : Environmental Microbiology and Environmental Microbiology Reports
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: 4 (7) Sequence Number: - Start / End Page: 375 - 382 Identifier: ISSN: 1462-2912
CoNE: https://pure.mpg.de/cone/journals/resource/959328105031