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  Azoarcus grass endophytes contribute fixed nitrogen to the plant in an unculturable state

Hurek, T., Handley, L. L., Reinhold-Hurek, B., & Piche, Y. (2002). Azoarcus grass endophytes contribute fixed nitrogen to the plant in an unculturable state. Molecular Plant-Microbe Interactions, 15(3), 233-242.

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Hurek, T.1, Author           
Handley, L. L., Author
Reinhold-Hurek, B., Author
Piche, Y., Author
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1Department of Symbiosis, Max Planck Institute for Marine Microbiology, Max Planck Society, ou_2481699              

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 Abstract: The extent to which the N-2-fixing bacterial endophyte Azoarcus sp. strain BH72 in the rhizosphere of Kallar grass can provide fixed nitrogen to the plant was assessed by evaluating inoculated plants grown in the greenhouse and uninoculated plants taken from the natural environment. The inoculum consisted of either wild-type bacteria or nifK(-) mutant strain BHNKD4. In N-2-deficient conditions, plants inoculated with strain BH72 (N2-fixing test plants) grew better and accumulated more nitrogen with a lower sigma(15)N signature after 8 months than did plants inoculated with the mutant strain (non-N-2- rixing control plants). Polyadenylated or polymerase chain reaction-amplified BH72 nifH transcripts were retrieved from test but not from control plants. BH72 nifH transcripts were abundant. The inocula could not be reisolated. These results indicate that Azoarcus sp. BH72 can contribute combined N-2 to the plant in an unculturable state. Abundant BH72 nifH transcripts were detected also in uninoculated plants taken from the natural environment, from which Azoarcus sp. BH72 also could not be isolated. Quantification of nitrogenase gene transcription indicated a high potential of strain BH72 for biological N2, fixation in association with roots. Phylogenetic analysis of nitrogenase sequences predicted that uncultured grass endophytes including Azoarcus spp. are ecologically dominant and play an important role in N-2-fixation in natural grass ecosystems.

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Language(s): eng - English
 Dates: 2002-03
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 13959
ISI: 000174407800007
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Title: Molecular Plant-Microbe Interactions
Source Genre: Journal
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Publ. Info: St. Paul, MN : APS Press
Pages: - Volume / Issue: 15 (3) Sequence Number: - Start / End Page: 233 - 242 Identifier: ISSN: 0894-0282
CoNE: https://pure.mpg.de/cone/journals/resource/954925558505