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  Proteasome isoforms exhibit only quantitative differences in cleavage and epitope generation.

Mishto, M., Liepe, J., Textoris-Taube, K., Keller, C., Henklein, P., Weberruss, M., et al. (2014). Proteasome isoforms exhibit only quantitative differences in cleavage and epitope generation. European Journal of Immunology, 44(12), 3508-3521. doi:10.1002/eji.201444902.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-FFF7-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-FFF9-A
Genre: Journal Article

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 Creators:
Mishto, M., Author
Liepe, J.1, Author              
Textoris-Taube, K., Author
Keller, C., Author
Henklein, P., Author
Weberruss, M., Author
Dahlmann, B., Author
Enenkel, C., Author
Voigt, A., Author
Kuckelkorn, U., Author
Stumpf, M. P. H., Author
Kloetzel, P. M., Author
Affiliations:
1Research Group of Quantitative and System Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_2466694              

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Free keywords: Antigen presentation; Immunoproteasome; MHC class I restricted epitopes; Proteasome; Proteolysis
 Abstract: Immunoproteasomes are considered to be optimised to process Ags and to alter the peptide repertoire by generating a qualitatively different set of MHC class I epitopes. Whether the immunoproteasome at the biochemical level, influence the quality rather than the quantity of the immuno-genic peptide pool is still unclear. Here, we quantified the cleavage-site usage by human standard- and immunoproteasomes, and proteasomes from immuno-subunit-deficient mice, as well as the peptides generated from model polypeptides. We show in this study that the different proteasome isoforms can exert significant quantitative differences in the cleavage-site usage and MHC class I restricted epitope production. However, independent of the proteasome isoform and substrates studied, no evidence was obtained for the abolishment of the specific cleavage-site usage, or for differences in the quality of the peptides generated. Thus, we conclude that the observed differences in MHC class I restricted Ag presentation between standard- and immunoproteasomes are due to quantitative differences in the proteasome-generated antigenic peptides.

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Language(s): eng - English
 Dates: 2014-11-202014-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/eji.201444902
 Degree: -

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Title: European Journal of Immunology
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 44 (12) Sequence Number: - Start / End Page: 3508 - 3521 Identifier: -