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  Carcinogen-induced DNA repair in nucleotide-permeable Escherichia coli cells. Induction of DNA repair by the carcinogens methyl and ethyl nitrosourea and methyl methanesulfonate

Thielmann, H. W., Vosberg, H.-P., & Reygers, U. (1975). Carcinogen-induced DNA repair in nucleotide-permeable Escherichia coli cells. Induction of DNA repair by the carcinogens methyl and ethyl nitrosourea and methyl methanesulfonate. European Journal of Biochemistry, 56(2), 433-447. doi:10.1111/j.1432-1033.1975.tb02250.x.

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Genre: Zeitschriftenartikel
Alternativer Titel : Carcinogen‐Induced DNA Repair in Nucleotide‐Permeable Escherichia coli Cells
Alternativer Titel : Induction of DNA Repair by the Carcinogens Methyl and Ethyl Nitrosourea and Methyl Methanesulfonate

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EurJBiochem_56_1975_433.pdf (beliebiger Volltext), 2MB
 
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https://doi.org/10.1111/j.1432-1033.1975.tb02250.x (beliebiger Volltext)
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Urheber

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 Urheber:
Thielmann, Heinz Walter1, Autor           
Vosberg, Hans-Peter2, Autor           
Reygers, Ursula1, Autor           
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Zusammenfassung: Ether-permeabilized (nucleotide-permeable) cells of Escherichia coli show excision repair of their DNA after having been exposed to the carcinogens N-methyl-N-nitrosourea (MeNOUr), N-ethyl-N-nitrosourea (EtNOUr) and methyl methanesulfonate (MeSO2OMe) which are known to bind covalently to DNA. Defect mutations in genes uvrA, uvrB, uvrC, recA, recB, recC and rep did not inhibit this excision repair. Enzymic activities involved in this repair were identified by measuring size reduction of DNA, DNA degradation to acid-soluble nucleotides and repair polymerization. 1. In permeabilized cells methyl and ethyl nitrosourea induced endonucleolytic cleavage of endogenous DNA, as determined by size reduction of denatured DNA in neutral and alkaline sucrose gradients. An enzymic activity from E. coli K-12 cell extracts was purified (greater than 2000-fold) and was found to cleave preferentially methyl-nitrosourea-treated DNA and to convert the methylated supercoiled DNA duplex (RFI) of phage phiX 174 into the nicked circular form. 2. Degradation of alkylated cellular DNA to acid solubility was diminished in a mutant lacking the 5' leads to 3' exonucleolytic activity of DNA polymerase I but was not affected in a mutant which lacked the DNA polymerizing but retained the 5' leads 3' exonucleolytic activity of DNA polymerase I. 3. An easily measurable effect is carcinogen-induced repair polymerization, making it suitable for detection of covalent binding of carcinogens and potentially carcinogenic compounds.

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Sprache(n): eng - English
 Datum: 1974-12-231975-03-282008-06-281975-08
 Publikationsstatus: Erschienen
 Seiten: 15
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
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Quelle 1

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Titel: European Journal of Biochemistry
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Seiten: - Band / Heft: 56 (2) Artikelnummer: - Start- / Endseite: 433 - 447 Identifikator: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040