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  CYP79 P450 monooxygenases in gymnosperms: CYP79A118 is associated with the formation of taxiphyllin in Taxus baccata

Luck, K., Jia, Q., Huber, M., Handrick, V., Wong, G. K., Nelson, D. R., et al. (2017). CYP79 P450 monooxygenases in gymnosperms: CYP79A118 is associated with the formation of taxiphyllin in Taxus baccata. Plant Molecular Biology, 95(1-2), 169-180. doi:10.1007/s11103-017-0646-0.

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http://dx.doi.org/10.1007/s11103-017-0646-0 (Publisher version)
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 Creators:
Luck, Katrin1, Author              
Jia, Qidong, Author
Huber, Meret1, 2, Author              
Handrick, Vinzenz1, 2, Author              
Wong, Gane Ka‑Shu, Author
Nelson, David R., Author
Chen, Feng, Author
Gershenzon, Jonathan1, Author              
Köllner, Tobias G.1, Author              
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Key message Conifers contain P450 enzymes from the CYP79 family that are involved in cyanogenic glycoside biosynthesis. Abstract Cyanogenic glycosides are secondary plant compounds that are widespread in the plant kingdom. Their biosynthesis starts with the conversion of aromatic or aliphatic amino acids into their respective aldoximes, catalysed by N-hydroxylating cytochrome P450 monooxygenases (CYP) of the CYP79 family. While CYP79s are well known in angiosperms, their occurrence in gymnosperms and other plant divisions containing cyanogenic glycoside-producing plants has not been reported so far. We screened the transcriptomes of 72 conifer species to identify putative CYP79 genes in this plant division. From the seven resulting fulllength genes, CYP79A118 from European yew (Taxus baccata) was chosen for further characterization. Recombinant CYP79A118 produced in yeast was able to convert l-tyrosine, l-tryptophan, and l-phenylalanine into p-hydroxyphenylacetaldoxime, indole-3-acetaldoxime, and phenylacetaldoxime, respectively. However, the kinetic parameters of the enzyme and transient expression of CYP79A118 in Nicotiana benthamiana indicate that l-tyrosine is the preferred substrate in vivo. Consistent with these findings, taxiphyllin, which is derived from l-tyrosine, was the only cyanogenic glycoside found in the different organs of T. baccata. Taxiphyllin showed highest accumulation in leaves and twigs, moderate accumulation in roots, and only trace accumulation in seeds and the aril. Quantitative real-time PCR revealed that CYP79A118 was expressed in plant organs rich in taxiphyllin. Our data show that CYP79s represent an ancient family of plant P450s that evolved prior to the separation of gymnosperms and angiosperms. CYP79A118 from T. baccata has typical CYP79 properties and its substrate specificity and spatial gene expression pattern suggest that the enzyme contributes to the formation of taxiphyllin in this plant species.

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 Dates: 2017-08-022017-08-09
 Publication Status: Published online
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 Identifiers: Other: GER489
DOI: 10.1007/s11103-017-0646-0
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Title: Plant Molecular Biology
  Other : Plant Mol. Biol.
Source Genre: Journal
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Publ. Info: The Hague : Martinus Nijhoff/Dr. W. Junk
Pages: - Volume / Issue: 95 (1-2) Sequence Number: - Start / End Page: 169 - 180 Identifier: ISSN: 0167-4412
CoNE: https://pure.mpg.de/cone/journals/resource/954925482643