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  Structural Changes in Sarcoplasmic Reticulum Membrane induced by SH Reagents

Dupont, Y., & Hasselbach, W. (1973). Structural Changes in Sarcoplasmic Reticulum Membrane induced by SH Reagents. Nature New Biology, 246, 41-44. doi:10.1038/newbio246041a0.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-0513-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-0514-2
Genre: Journal Article

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NatNewBiol_246_1973_41.pdf (Any fulltext), 421KB
 
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http://www.nature.com/nature-newbio/journal/v246/n150/pdf/newbio246041a0.pdf (Any fulltext)
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Dupont, Y., Author
Hasselbach, Wilhelm1, Author           
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1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: Using sarcoplasmic reticulum (SR) membranes, stacked and orientated by centrifugation, Dupont, Harrison and Hasselbach1 demonstrated that it was possible to record Bragg reflections to 15 Å−1 resolution. The phases of the reflections and the electron density profiles were obtained to a resolution of 30 Å. In an attempt to label the calcium pump protein with heavy atoms very dramatic changes were observed in the X-ray diffraction pattern when organo-mereurials or other SH reagents were bound to the ATPase. These changes seem to have been due to large structural modifications of the membrane.

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Language(s): eng - English
 Dates: 1973-07-061973-09-201973-11-14
 Publication Status: Issued
 Pages: 4
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/newbio246041a0
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Title: Nature New Biology
Source Genre: Journal
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Publ. Info: London : Macmillan
Pages: - Volume / Issue: 246 Sequence Number: - Start / End Page: 41 - 44 Identifier: ISSN: 0090-0028