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  Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

Weiss, M., Frohnmayer, J. P., Benk, L. T., Haller, B., Janiesch, J.-W., Heitkamp, T., et al. (2018). Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nature Materials, 17(1), 89-96. doi:10.1038/nmat5005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-173F-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-62AD-F
Genre: Journal Article

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 Creators:
Weiss, Marian, Author
Frohnmayer, Johannes Patrick, Author
Benk, Lucia Theresa, Author
Haller, Barbara, Author
Janiesch, Jan-Willi, Author
Heitkamp, Thomas, Author
Borsch, Michael, Author
Lira, Rafael B.1, Author              
Dimova, Rumiana1, Author              
Lipowsky, Reinhard2, Author              
Bodenschatz, Eberhard, Author
Baret, Jean-Christophe, Author
Vidakovic-Koch, Tanja, Author
Sundmacher, Kai, Author
Platzman, Ilia, Author
Spatz, Joachim P., Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Reinhard Lipowsky, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863327              

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 Abstract: Compartments for the spatially and temporally controlled assembly of biological processes are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid-based protocells lack the mechanical and chemical stability to allow their manipulation into a complex and fully functional synthetic cell. Here, we present a high-throughput microfluidic method to generate stable, defined sized liposomes termed /`droplet-stabilized giant unilamellar vesicles (dsGUVs)[rsquor]. The enhanced stability of dsGUVs enables the sequential loading of these compartments with biomolecules, namely purified transmembrane and cytoskeleton proteins by microfluidic pico-injection technology. This constitutes an experimental demonstration of a successful bottom-up assembly of a compartment with contents that would not self-assemble to full functionality when simply mixed together. Following assembly, the stabilizing oil phase and droplet shells are removed to release functional self-supporting protocells to an aqueous phase, enabling them to interact with physiologically relevant matrices.

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 Dates: 2017-10-162018-01
 Publication Status: Published in print
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 Identifiers: DOI: 10.1038/nmat5005
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Title: Nature Materials
  Abbreviation : Nat. Mater.
Source Genre: Journal
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Publ. Info: London, UK : Nature Pub. Group
Pages: - Volume / Issue: 17 (1) Sequence Number: - Start / End Page: 89 - 96 Identifier: ISSN: 1476-1122