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  Analyzing the spatial positioning of nuclei in polynuclear giant cells

Stange, M., Hintsche, M., Sachse, K., Gerhardt, M., Valleriani, A., & Beta, C. (2017). Analyzing the spatial positioning of nuclei in polynuclear giant cells. Journal of Physics D: Applied Physics, 50(46): 464001. doi:10.1088/1361-6463/aa8da0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-1741-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-9616-0
Genre: Journal Article

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 Creators:
Stange, Maike, Author
Hintsche, Marius, Author
Sachse, Kirsten, Author
Gerhardt, Matthias, Author
Valleriani, Angelo1, Author              
Beta, Carsten, Author
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1Angelo Valleriani, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863324              

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 Abstract: How cells establish and maintain a well-defined size is a fundamental question of cell biology. Here we investigated to what extent the microtubule cytoskeleton can set a predifined cell size, independent of an enclosing cell membrane. We used electropulse-induced cell fusion to form giant multinuclear cells of the social amoeba Dictyostelium discoideum. Based on dual-color confocal imaging of cells that expressed fluorescent markers for the cell nucleus and the microtubules, we determined the subcellular distributions of nuclei and centrosomes in the giant cells. Our two- and three-dimensional imaging results showed that the positions of nuclei in giant cells do not fall onto a regular lattice. However, a comparison with model predictions for random positioning showed that the subcellular arrangement of nuclei maintains a low but still detectable degree of ordering. This can be explained by the steric requirements of the microtubule cytoskeleton, as confirmed by the effect of a microtubule degrading drug.13;

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 Dates: 2017-09-192017-10-24
 Publication Status: Published in print
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 Identifiers: DOI: 10.1088/1361-6463/aa8da0
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Title: Journal of Physics D: Applied Physics
  Abbreviation : J. Phys. D: Appl. Phys.
Source Genre: Journal
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Publ. Info: Bristol : IOP Publishing
Pages: - Volume / Issue: 50 (46) Sequence Number: 464001 Start / End Page: - Identifier: ISSN: 0022-3727