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  STED imaging in Drosophila brain slices

Fendl, S., Pujol-Marti, J., Ryan, J., Borst, A., & Kasper, R. (2017). STED imaging in Drosophila brain slices. In Y. Markaki, & H. Harz (Eds.), Light Microscopy (pp. 143-150). New York, NY: Humana Press.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-0A9C-A Version Permalink: https://hdl.handle.net/21.11116/0000-000A-03A9-4
Genre: Book Chapter

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 Creators:
Fendl, Sandra1, Author           
Pujol-Marti, Jesus1, Author           
Ryan, Joel, Author
Borst, Alexander1, Author           
Kasper, Robert2, Author           
Affiliations:
1Department: Circuits-Computation-Models / Borst, MPI of Neurobiology, Max Planck Society, ou_1113548              
2MPI of Neurobiology, Max Planck Society, ou_1110547              

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Free keywords: MICROSCOPY; SYNAPSES; SYSTEM; TOOLS; FLYBiochemistry & Molecular Biology; Microscopy; STED; Drosophila melanogaster; Immunofluorescence; Cryostat sectioning; Brain slice;
 Abstract: Super-resolution microscopy is a very powerful tool to investigate fine cellular structures and molecular arrangements in biological systems. For instance, stimulated emission depletion (STED) microscopy has been successfully used in recent years to investigate the arrangement and colocalization of different protein species in cells in culture and on the surface of specimens. However, because of its extreme sensitivity to light scattering, super-resolution imaging deep inside tissues remains a challenge. Here, we describe the preparation of thin slices from the fruit fly (Drosophila melanogaster) brain, subsequent immunolabeling and imaging with STED microscopy. This protocol allowed us to image small dendritic branches from neurons located deep in the fly brain with improved resolution compared with conventional light microscopy.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000411868600011
DOI: 10.1007/978-1-4939-6810-7_10
 Degree: -

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Title: Light Microscopy
  Subtitle : Methods and Protocols
  Other : Methods in Molecular Biology
Source Genre: Book
 Creator(s):
Markaki, Y., Editor
Harz, H., Editor
Affiliations:
-
Publ. Info: New York, NY : Humana Press
Pages: - Volume / Issue: 1563 Sequence Number: - Start / End Page: 143 - 150 Identifier: ISBN: 978-1-4939-6810-7; 978-1-4939-6808-4

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Alternative Title : Methods Mol. Biol.
Source Genre: Series
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1563 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745