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  Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

Weiss, M., Frohnmayer, J. P., Benk, L. T., Haller, B., Janiesch, J.-W., Heitkamp, T., et al. (2018). Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nature Materials, 17, 89-96. doi:10.1038/nmat5005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-0AA2-B Version Permalink: http://hdl.handle.net/21.11116/0000-0002-CCE9-2
Genre: Journal Article

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 Creators:
Weiss, Marian1, Author              
Frohnmayer, Johannes Patrick1, 2, Author              
Benk, Lucia Theresa1, Author              
Haller, Barbara1, Author              
Janiesch, Jan-Willi1, 2, Author              
Heitkamp, Thomas, Author
Börsch, Michael, Author
Lira, Rafael B., Author
Dimova, Rumiana, Author
Lipowsky, Reinhard, Author
Bodenschatz, Eberhard, Author
Baret, Jean-Christophe, Author
Vidakovic-Koch, Tanja, Author
Sundmacher, Kai, Author
Platzman, Ilia1, 2, Author              
Spatz, Joachim P.1, 2, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: Biomaterials; Lab-on-a-chip; Microfluidics; Synthetic biology
 Abstract: Compartments for the spatially and temporally controlled assembly of biological processes are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid-based protocells lack the mechanical and chemical stability to allow their manipulation into a complex and fully functional synthetic cell. Here, we present a high-throughput microfluidic method to generate stable, defined sized liposomes termed ‘droplet-stabilized giant unilamellar vesicles (dsGUVs)’. The enhanced stability of dsGUVs enables the sequential loading of these compartments with biomolecules, namely purified transmembrane and cytoskeleton proteins by microfluidic pico-injection technology. This constitutes an experimental demonstration of a successful bottom-up assembly of a compartment with contents that would not self-assemble to full functionality when simply mixed together. Following assembly, the stabilizing oil phase and droplet shells are removed to release functional self-supporting protocells to an aqueous phase, enabling them to interact with physiologically relevant matrices.

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Language(s): eng - English
 Dates: 2016-06-062017-09-122017-10-162018-01-01
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nmat5005
 Degree: -

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Title: Nature Materials
  Abbreviation : Nat. Mater.
Source Genre: Journal
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Publ. Info: London, UK : Nature Pub. Group
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 89 - 96 Identifier: ISSN: 1476-1122
CoNE: /journals/resource/111054835734000