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  Isolation and Characterization of Metallosphaera Turreted Icosahedral Virus, a Founding Member of a New Family of Archaeal Viruses

Wagner, C., Reddy, V., Asturias, F., Khoshouei, M., Johnson, J. E., Manrique, P., et al. (2017). Isolation and Characterization of Metallosphaera Turreted Icosahedral Virus, a Founding Member of a New Family of Archaeal Viruses. Journal of Virology, 91(20): UNSP e00925-17. doi:10.1128/JVI.00925-17.

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 Creators:
Wagner, Cassia1, Author
Reddy, Vijay1, Author
Asturias, Francisco1, Author
Khoshouei, Maryam2, Author              
Johnson, John E.1, Author
Manrique, Pilar1, Author
Munson-McGee, Jacob1, Author
Baumeister, Wolfgang2, Author              
Lawrence, C. Martin2, Author              
Young, Mark J.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: TRANSMISSION ELECTRON-MICROSCOPY; CRYOELECTRON TOMOGRAPHY; HALOARCULA-HISPANICA; VIRAL DIVERSITY; SEQUENCE DATA; PHASE PLATE; HOT-SPRINGS; DNA; PROTEINS; ORGANIZATIONVirology; Archaea; archaeal virus; crenarchaeal; cryo-electron microscopy; environmental virology; extremophiles; viruses in extreme environments;
 Abstract: Our understanding of archaeal virus diversity and structure is just beginning to emerge. Here we describe a new archaeal virus, tentatively named Metallosphaera turreted icosahedral virus (MTIV), that was isolated from an acidic hot spring in Yellowstone National Park, USA. Two strains of the virus were identified and were found to replicate in an archaeal host species closely related to Metallosphaera yellowstonensis. Each strain encodes a 9.8-to 9.9-kb linear double-stranded DNA (dsDNA) genome with large inverted terminal repeats. Each genome encodes 21 open reading frames (ORFs). The ORFs display high homology between the strains, but they are quite distinct from other known viral genes. The 70-nm-diameter virion is built on a T = 28 icosahedral lattice. Both single particle cryoelectron microscopy and cryotomography reconstructions reveal an unusual structure that has 42 turret-like projections: 12 pentameric turrets positioned on the icosahedral 5-fold axes and 30 turrets with apparent hexameric symmetry positioned on the icosahedral 2-fold axes. Both the virion structural properties and the genome content support MTIV as the founding member of a new family of archaeal viruses. IMPORTANCE Many archaeal viruses are quite different from viruses infecting bacteria and eukaryotes. Initial characterization of MTIV reveals a virus distinct from other known bacterial, eukaryotic, and archaeal viruses; this finding suggests that viruses infecting Archaea are still an understudied group. As the first known virus infecting a Metallosphaera sp., MTIV provides a new system for exploring archaeal virology by examining host-virus interactions and the unique features of MTIV structure-function relationships. These studies will likely expand our understanding of virus ecology and evolution.

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Language(s): eng - English
 Dates: 2017-08-02
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000411845000023
DOI: 10.1128/JVI.00925-17
 Degree: -

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Title: Journal of Virology
Source Genre: Journal
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Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 91 (20) Sequence Number: UNSP e00925-17 Start / End Page: - Identifier: ISSN: 0022-538X
CoNE: https://pure.mpg.de/cone/journals/resource/954925419045