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  The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization

Freeman Rosenzweig, E. S., Xu, B., Kuhn Cuellar, L., Martinez-Sanchez, A., Schaffer, M., Strauss, M., et al. (2017). The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization. Cell, 171(1), 148-162.e19. doi:10.1016/j.cell.2017.08.008.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0000-780F-A Version Permalink: http://hdl.handle.net/21.11116/0000-0000-7810-7
Genre: Journal Article

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 Creators:
Freeman Rosenzweig, Elizabeth S.1, Author
Xu, Bin1, Author
Kuhn Cuellar, Luis2, Author              
Martinez-Sanchez, Antonio2, Author              
Schaffer, Miroslava2, Author              
Strauss, Mike3, Author              
Cartwright, Heather N.1, Author
Ronceray, Pierre1, Author
Plitzko, Jürgen M.2, Author              
Förster, Friedrich2, Author              
Wingreen, Ned S.1, Author
Engel, Benjamin D.2, Author              
Mackinder, Luke C. M.1, Author
Jonikas, Martin C.1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
3Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: CHLAMYDOMONAS-REINHARDTII; RUBISCO ACTIVASE; DISORDERED PROTEINS; PYRENOID FORMATION; GREEN-ALGAE; CARBOXYSOMES; TOMOGRAPHY; CO2; CARBOXYLASE/OXYGENASE; PHOSPHORYLATIONBiochemistry & Molecular Biology; Cell Biology;
 Abstract: Approximately 30%-40% of global CO2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number'' effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.

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Language(s): eng - English
 Dates: 2017-09-212017
 Publication Status: Published in print
 Pages: 34
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 171 (1) Sequence Number: - Start / End Page: 148 - 162.e19 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183