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  Isolation of Mitochondria from Saccharomyces cerevisiae

Izawa, T., & Unger, A.-K. (2017). Isolation of Mitochondria from Saccharomyces cerevisiae. In D. Mokranjac, & F. Perocchi (Eds.), Mitochondria (pp. 33-42). New York, NY: Humana Press.

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 Creators:
Izawa, Toshiaki1, Author              
Unger, Ann-Katrin2, Author
Affiliations:
1Neupert, Walter / Structure and Function of Mitochondria, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565163              
2external, ou_persistent22              

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Free keywords: YEAST; TIM50; PURIFICATION; TRANSLOCASE; COMPLEX; OUTERYeast culture media; Spheroplasts; Homogenization; Cellular fractionation; Isolation and purification of mitochondria;
 Abstract: The budding yeast Saccharomyces cerevisiae is an important model organism to study cellular structure and function. Due to its excellent accessibility to genetics and biochemical and microscopic analyses, studies with yeast have provided fundamental insights into mitochondrial biology. Yeast offers additional advantages because it can grow under fermenting conditions when oxidative phosphorylation is not obligatory and because the majority of mitochondrial structure and function are largely conserved during evolution. Isolation of mitochondria is an important technique for mitochondrial studies. This chapter focuses on procedures for the isolation and purification of intact yeast mitochondria that can be used for numerous functional assays as well as for analyses of mitochondrial ultrastructure.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Mitochondria
  Subtitle : Practical Protocols
  Subtitle : Methods in Molecular Biology, vol 1567
Source Genre: Book
 Creator(s):
Mokranjac, D., Editor
Perocchi, F., Editor
Affiliations:
-
Publ. Info: New York, NY : Humana Press
Pages: - Volume / Issue: 1567 Sequence Number: - Start / End Page: 33 - 42 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-6824-4; 978-1-4939-6822-0

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Abbreviation : MIMB
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: New York : Humana Press
Pages: - Volume / Issue: 1567 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745