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  Analysis of Yeast Mitochondria by Electron Microscopy

Unger, A.-K., Geimer, S., Harner, M., Neupert, W., & Westermann, B. (2017). Analysis of Yeast Mitochondria by Electron Microscopy. In D. Mokranjac, & F. Perocchi (Eds.), Mitochondria (2nd ed., pp. 293-314). New York, NY: Humana Press.

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 Creators:
Unger, Ann-Katrin1, Author
Geimer, Stefan1, Author
Harner, Max1, Author
Neupert, Walter2, Author              
Westermann, Benedikt1, Author
Affiliations:
1external, ou_persistent22              
2Neupert, Walter / Structure and Function of Mitochondria, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565163              

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Free keywords: SACCHAROMYCES-CEREVISIAE; MEMBRANE ORGANIZATION; MOTOR-PROTEIN; CONTACT SITE; CRISTAE; ARCHITECTURE; DYNAMICS; CELL; BIOGENESIS; TRANSPORTImmunoelectron microscopy; Mitochondria; Organelle architecture; Saccharomyces cerevisiae; Tokuyasu cryosectioning; Transmission electron microscopy;
 Abstract: Budding yeast Saccharomyces cerevisiae represents a widely used model organism for the study of mitochondrial biogenesis and architecture. Electron microscopy is an essential tool in the analysis of cellular ultrastructure and the precise localization of proteins to organellar subcompartments. We provide here detailed protocols for the analysis of yeast mitochondria by transmission electron microscopy: (1) chemical fixation and Epon embedding of yeast cells and isolated mitochondria, and (2) cryosectioning and immunolabeling of yeast cells and isolated mitochondria according to the Tokuyasu method.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Mitochondria
  Subtitle : Practical Protocols
  Other : Methods in Molecular Biology
Source Genre: Book
 Creator(s):
Mokranjac, D.1, Editor
Perocchi, F.1, Editor
Affiliations:
1 External Organizations, ou_persistent22            
Publ. Info: New York, NY : Humana Press, 2nd ed.
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 293 - 314 Identifier: ISSN: 1064-3745
ISBN: 978-1-4939-6824-4; 978-1-4939-6822-0

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Title: Methods in Molecular Biology
  Alternative Title : METHODS MOL BIOL
  Alternative Title : Methods Mol. Biol.
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1567 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745