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  Mechanism of transcription anti-termination in human mitochondria.

Hillen, H. S., Parshin, A. V., Agaronyan, K., Morozov, Y. I., Graber, J. J., Chernev, A., et al. (2017). Mechanism of transcription anti-termination in human mitochondria. Cell, 171(5), 1082-1093. doi:10.1016/j.cell.2017.09.035.

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 Creators:
Hillen, H. S.1, Author           
Parshin, A. V., Author
Agaronyan, K., Author
Morozov, Y. I., Author
Graber, J. J., Author
Chernev, A.2, Author           
Schwinghammer, K.3, Author           
Urlaub, H.2, Author           
Anikin, M., Author
Cramer, P.3, Author           
Temiakov, D., Author
Affiliations:
1Research Group Structure and Function of Molecular Machines, MPI for Biophysical Chemistry, Max Planck Society, ou_3265856              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              
3Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: mitochondria, transcription, POLRMT, TEFM, TFAM, TFB2M, replication, G-quadruplex, elongation
 Abstract: In human mitochondria, transcription termination events at a G-quadruplex region near the replication origin are thought to drive replication of mtDNA by generation of an RNA primer. This process is suppressed by a key regulator of mtDNA—the transcription factor TEFM. We determined the structure of an anti-termination complex in which TEFM is bound to transcribing mtRNAP. The structure reveals interactions of the dimeric pseudonuclease core of TEFM with mobile structural elements in mtRNAP and the nucleic acid components of the elongation complex (EC). Binding of TEFM to the DNA forms a downstream “sliding clamp,” providing high processivity to the EC. TEFM also binds near the RNA exit channel to prevent formation of the RNA G-quadruplex structure required for termination and thus synthesis of the replication primer. Our data provide insights into target specificity of TEFM and mechanisms by which it regulates the switch between transcription and replication of mtDNA

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Language(s): eng - English
 Dates: 2017-10-122017-11-16
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.cell.2017.09.035
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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 171 (5) Sequence Number: - Start / End Page: 1082 - 1093 Identifier: -