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  Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics

Weiss, M., Frohnmayer, J. P., Benk, L. T., Haller, B., Janiesch, J. W., Heitkamp, T., et al. (2018). Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nature Materials, 17(1), 89-96. doi:10.1038/nmat5005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-194B-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-71F9-7
Genre: Journal Article

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 Creators:
Weiss, M., Author
Frohnmayer, J. P., Author
Benk, L. T., Author
Haller, B., Author
Janiesch, J. W., Author
Heitkamp, T., Author
Börsch, M., Author
Lira, R. B., Author
Dimova, R., Author
Lipowsky, R., Author
Bodenschatz, Eberhard1, Author              
Baret, Jean-Christophe2, Author              
Vidakovic-Koch, T., Author
Sundmacher, K., Author
Platzmann, I., Author
Spatz, J. P., Author
Affiliations:
1Laboratory for Fluid Dynamics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063287              
2Max Planck Research Group Droplets, Membranes and Interfaces, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society, ou_2063290              

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 Abstract: Compartments for the spatially and temporally controlled assembly of biological processes are essential towards cellular life. Synthetic mimics of cellular compartments based on lipid-based protocells lack the mechanical and chemical stability to allow their manipulation into a complex and fully functional synthetic cell. Here, we present a high-throughput microfluidic method to generate stable, defined sized liposomes termed ‘droplet-stabilized giant unilamellar vesicles (dsGUVs)’. The enhanced stability of dsGUVs enables the sequential loading of these compartments with biomolecules, namely purified transmembrane and cytoskeleton proteins by microfluidic pico-injection technology. This constitutes an experimental demonstration of a successful bottom-up assembly of a compartment with contents that would not self-assemble to full functionality when simply mixed together. Following assembly, the stabilizing oil phase and droplet shells are removed to release functional self-supporting protocells to an aqueous phase, enabling them to interact with physiologically relevant matrices.

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Language(s): eng - English
 Dates: 2017-10-162018-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nmat5005
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Title: Nature Materials
Source Genre: Journal
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Pages: - Volume / Issue: 17 (1) Sequence Number: - Start / End Page: 89 - 96 Identifier: -