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  Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides.

Carrocci, T. J., Lohe, L., Ashton, M. J., Höbartner, C., & Hoskins, A. A. (2017). Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides. Chemical Communications, 53(88), 11992-11995. doi:10.1039/c7cc06703h.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-198D-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-2CBA-1
Genre: Journal Article

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2492780.pdf (Publisher version), 3MB
 
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 Creators:
Carrocci, T. J., Author
Lohe, L., Author
Ashton, M. J., Author
Höbartner, C.1, Author              
Hoskins, A. A., Author
Affiliations:
1Research Group of Nucleic Acid Chemistry, MPI for biophysical chemistry, Max Planck Society, ou_578605              

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 Abstract: The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.

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Language(s): eng - English
 Dates: 2017-09-292017
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1039/c7cc06703h
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Title: Chemical Communications
Source Genre: Journal
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Pages: - Volume / Issue: 53 (88) Sequence Number: - Start / End Page: 11992 - 11995 Identifier: -