Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted 
fluorescent proteins

Laviv, Tal; Kim, Benjamin B.; Chu, Jun; Lam, Amy J.; Lin, Michael Z.; Yasuda, Ryohei

doi:10.1038/nmeth.4046

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Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins

Laviv, T., Kim, B. B., Chu, J., Lam, A. J., Lin, M. Z., & Yasuda, R. (2016). Simultaneous dual-color fluorescence lifetime imaging with novel red-shifted fluorescent proteins. Nature Methods, advance online publication, 989-992. doi:10.1038/nmeth.4046.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2AE0-A Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002E-2AE1-8

Genre: Zeitschriftenartikel

Alternativer Titel : Nature Methods

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Urheber:
Laviv, Tal¹, Autor
Kim, Benjamin B.², Autor
Chu, Jun², Autor
Lam, Amy J.², Autor
Lin, Michael Z.², Autor
Yasuda, Ryohei¹, Autor

Affiliations:
1Max Planck Florida Institute for Neuroscience, Max Planck Society, One Max Planck Way, Jupiter FL 33458, USA, ou_1950288
2External Organizations, ou_persistent22

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Schlagwörter: Cell signalling, Fluorescent proteins, Molecular neuroscience

Zusammenfassung: We describe a red-shifted fluorescence resonance energy transfer (FRET) pair optimized for dual-color fluorescence lifetime imaging (FLIM). This pair utilizes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which has a large Stokes shift and a monoexponential fluorescence lifetime decay. When used together with EGFP-based biosensors, the new pair enables simultaneous imaging of the activities of two signaling molecules in single dendritic spines undergoing structural plasticity.