English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration.

Zobel, M., Goen, T., Belov, V. N., & Klotz, K. (2017). Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration. Analytical and Bioanalytical Chemistry, 409(29), 6861-6872. doi:10.1007/s00216-017-0651-9.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-2F59-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-2F5D-0
Genre: Journal Article

Files

show Files
hide Files
:
2502850.pdf (Publisher version), 749KB
 
File Permalink:
-
Name:
2502850.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2502850_Suppl.pdf (Supplementary material), 900KB
Name:
2502850_Suppl.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Zobel, M., Author
Goen, T., Author
Belov, V. N.1, Author              
Klotz , K., Author
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

Content

show
hide
Free keywords: Biomonitoring; Hydrolysis; Naphthalene; Glucuronide; Naphthol; Chemical stability
 Abstract: After environmental and occupational exposure to naphthalene, 1,2-dihydroxynaphthalene (1,2-DHN) was shown to be one major metabolite in human naphthalene metabolism. However, the instability of free 1,2-DHN complicates the reliable determination of this promising biomarker in urine. To solve this stability problem, glucuronide conjugates of 1,2-DHN and the corresponding isotopically labelled D-6-1,2-dihydroxynaphthalene (D-6-1,2-DHN) were synthesised and applied as reference material and internal standard in a gas chromatographic-tandem mass spectrometric (GC-MS/MS) method. The determination of 1- and 2-naphthol (1-MHN, 2-MHN) was included in the procedure to enable a comprehensive assessment of naphthalene metabolism and exposure. The results of the validation showed a high reliability and sensitivity of the method. The detection limits range from 0.05 to 0.16 mu g/L. Precision and repeatability were determined to range from 1.4 to 6.6% for all parameters. The simultaneous determination of 1- and 2-MHN as additional parameters besides 1,2-DHN enables the application of the method for further metabolism and kinetic studies on naphthalene. The use of glucuronide-derivative reference substances and the application of structurally matched isotopic-labelled internal standards for each substance guarantee a reliable quantification of the main naphthalene metabolites 1,2-DHN and 1- and 2-MHN.

Details

show
hide
Language(s): eng - English
 Dates: 2017-10-102017-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s00216-017-0651-9
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Analytical and Bioanalytical Chemistry
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 409 (29) Sequence Number: - Start / End Page: 6861 - 6872 Identifier: -