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  Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration.

Zobel, M., Goen, T., Belov, V. N., & Klotz, K. (2017). Reliable quantification of 1,2-dihydroxynaphthalene in urine using a conjugated reference compound for calibration. Analytical and Bioanalytical Chemistry, 409(29), 6861-6872. doi:10.1007/s00216-017-0651-9.

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 Urheber:
Zobel, M., Autor
Goen, T., Autor
Belov, V. N.1, Autor           
Klotz , K., Autor
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Schlagwörter: Biomonitoring; Hydrolysis; Naphthalene; Glucuronide; Naphthol; Chemical stability
 Zusammenfassung: After environmental and occupational exposure to naphthalene, 1,2-dihydroxynaphthalene (1,2-DHN) was shown to be one major metabolite in human naphthalene metabolism. However, the instability of free 1,2-DHN complicates the reliable determination of this promising biomarker in urine. To solve this stability problem, glucuronide conjugates of 1,2-DHN and the corresponding isotopically labelled D-6-1,2-dihydroxynaphthalene (D-6-1,2-DHN) were synthesised and applied as reference material and internal standard in a gas chromatographic-tandem mass spectrometric (GC-MS/MS) method. The determination of 1- and 2-naphthol (1-MHN, 2-MHN) was included in the procedure to enable a comprehensive assessment of naphthalene metabolism and exposure. The results of the validation showed a high reliability and sensitivity of the method. The detection limits range from 0.05 to 0.16 mu g/L. Precision and repeatability were determined to range from 1.4 to 6.6% for all parameters. The simultaneous determination of 1- and 2-MHN as additional parameters besides 1,2-DHN enables the application of the method for further metabolism and kinetic studies on naphthalene. The use of glucuronide-derivative reference substances and the application of structurally matched isotopic-labelled internal standards for each substance guarantee a reliable quantification of the main naphthalene metabolites 1,2-DHN and 1- and 2-MHN.

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Sprache(n): eng - English
 Datum: 2017-10-102017-11
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1007/s00216-017-0651-9
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Titel: Analytical and Bioanalytical Chemistry
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 409 (29) Artikelnummer: - Start- / Endseite: 6861 - 6872 Identifikator: -