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  Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature

Poreddy, S., Li, J., & Baldwin, I. T. (2017). Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature. BMC Plant Biology, 17: 199. doi:10.1186/s12870-017-1149-5.

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Poreddy, Spoorthi1, 2, Author              
Li, Jiancai1, Author              
Baldwin, Ian Thomas1, Author              
Affiliations:
1Department of Molecular Ecology, Prof. I. T. Baldwin, MPI for Chemical Ecology, Max Planck Society, ou_24029              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

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 Abstract: Background: Plant-mediated RNAi (PMRi) silencing of insect genes has enormous potential for crop protection, but whether it works robustly under field conditions, particularly with lepidopteran pests, remains controversial. Wild tobacco Nicotiana attenuata and cultivated tobacco (N. tabacum) (Solanaceae) is attacked by two closely related specialist herbivores Manduca sexta and M. quinquemaculata (Lepidoptera, Sphingidae). When M. sexta larvae attack transgenic N. attenuata plants expressing double-stranded RNA(dsRNA) targeting M. sexta’s midgut-expressed genes, the nicotine-ingestion induced cytochrome P450 monooxygenase (invert repeat (ir)CYP6B46-plants) and the lyciumoside-IV-ingestion induced β-glucosidase1 (irBG1-plants), these larval genes which are important for the larvae’s response to ingested host toxins, are strongly silenced. Results: Here we show that the PMRi procedure also silences the homologous genes in native M. quinquemaculata larvae feeding on irCYP6B46 and irBG1-transgenic N. attenuata plants in nature. The PMRi lines shared 98 and 96% sequence similarity with M. quinquemaculata homologous coding sequences, and CYP6B46 and BG1 transcripts were reduced by ca. 90 and 80%, without reducing the transcripts of the larvae’s most similar, potential off-target genes. Conclusions: We conclude that the PMRi procedure can robustly and specifically silence genes in native congeneric insects that share sufficient sequence similarity and with the careful selection of targets, might protect crops from attack by congeneric-groups of insect pests.

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 Dates: 2017-11-022017-11-132017
 Publication Status: Published in print
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 Identifiers: Other: ITB565
DOI: 10.1186/s12870-017-1149-5
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Title: BMC Plant Biology
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 17 Sequence Number: 199 Start / End Page: - Identifier: ISSN: 1471-2229
CoNE: https://pure.mpg.de/cone/journals/resource/111032787578000