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  A method for the acute and rapid degradation of endogenous proteins.

Clift, D., McEwan, W. A., Labzin, L. I., Konieczny, V., Mogessie, B., James, L. C., et al. (2017). A method for the acute and rapid degradation of endogenous proteins. Cell, 171(7), 1692-1706. doi:10.1016/j.cell.2017.10.033.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-3711-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-919F-2
Genre: Journal Article

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 Creators:
Clift, D., Author
McEwan, W. A., Author
Labzin, L. I., Author
Konieczny, V.1, Author              
Mogessie, B.1, Author              
James, L. C., Author
Schuh, M.1, Author              
Affiliations:
1Department of Meiosis, MPI for Biophysical Chemistry, Max Planck Society, ou_2205654              

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Free keywords: CRISPR/Cas9; RNAi; antibodies; cell division; macrophages; meiosis; oocytes; primary cells; protein degradation; protein knockdown
 Abstract: Methods for the targeted disruption of protein function have revolutionized science and greatly expedited the systematic characterization of genes. Two main approaches are currently used to disrupt protein function: DNA knockout and RNA interference, which act at the genome and mRNA level, respectively. A method that directly alters endogenous protein levels is currently not available. Here, we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without prior modification of the genome or mRNA. Trim-Away harnesses the cellular protein degradation machinery to remove unmodified native proteins within minutes of application. This rapidity minimizes the risk that phenotypes are compensated and that secondary, non-specific defects accumulate over time. Because Trim-Away utilizes antibodies, it can be applied to a wide range of target proteins using off-the-shelf reagents. Trim-Away allows the study of protein function in diverse cell types, including non-dividing primary cells where genome- and RNA-targeting methods are limited.

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Language(s): eng - English
 Dates: 2017-11-162017-12-14
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.cell.2017.10.033
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 171 (7) Sequence Number: - Start / End Page: 1692 - 1706 Identifier: -