Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Protein profiling of non-model plant Cuminum cyminum by gel-based proteomic approach.

Zaman, U., Urlaub, H., & Abbasi, A. (2018). Protein profiling of non-model plant Cuminum cyminum by gel-based proteomic approach. Phytochemical Analysis, 29(3), 242-249. doi:10.1002/pca.2738.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
2504297.pdf (Verlagsversion), 525KB
 
Datei-Permalink:
-
Name:
2504297.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt ( Max Planck Society (every institute); )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
2504297_Suppl.zip (Ergänzendes Material), 576KB
Name:
2504297_Suppl.zip
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/zip / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Zaman, U.1, Autor           
Urlaub, H.1, Autor           
Abbasi, A., Autor
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Cuminum cyminum; cumin; liquid chromatography-high resolution mass spectrometry; one-dimensional gel electrophoresis; plant proteomics; protein extraction
 Zusammenfassung: Introduction

Cumin (Cuminum cyminum), a popular spice has been widely used in traditional medicine to cure various ailments. Despite the existence of scientific literature about its pharmacological properties, no successful proteome profiling has yet been attempted.
Objective

To optimise extraction of cumin proteins and analyse its profile by shotgun proteomics, using one-dimensional electrophoresis coupled with nano-ESI-LC–MS/MS.
Methodology

As a first step, we have compared three extraction protocols for total proteins extraction from cumin. Extracted proteins were separated on one-dimensional gel and analysed by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose and Q Exactive HF mass spectrometer.
Results

Evaluation of extraction method revealed significant differences in protein yield and proteome composition between the three extracts. LC–MS/MS allowed identification of several proteins with functional significance in various biological processes.
Conclusion

This study provides identification of a large number of proteins and offers a molecular basis for future research on potential pharmacologically active cumin proteins.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2017-11-162018-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1002/pca.2738
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Phytochemical Analysis
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 29 (3) Artikelnummer: - Start- / Endseite: 242 - 249 Identifikator: -