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キーワード:
Cuminum cyminum; cumin; liquid chromatography-high resolution mass spectrometry; one-dimensional gel electrophoresis; plant proteomics; protein extraction
要旨:
Introduction
Cumin (Cuminum cyminum), a popular spice has been widely used in traditional medicine to cure various ailments. Despite the existence of scientific literature about its pharmacological properties, no successful proteome profiling has yet been attempted.
Objective
To optimise extraction of cumin proteins and analyse its profile by shotgun proteomics, using one-dimensional electrophoresis coupled with nano-ESI-LC–MS/MS.
Methodology
As a first step, we have compared three extraction protocols for total proteins extraction from cumin. Extracted proteins were separated on one-dimensional gel and analysed by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose and Q Exactive HF mass spectrometer.
Results
Evaluation of extraction method revealed significant differences in protein yield and proteome composition between the three extracts. LC–MS/MS allowed identification of several proteins with functional significance in various biological processes.
Conclusion
This study provides identification of a large number of proteins and offers a molecular basis for future research on potential pharmacologically active cumin proteins.
