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  Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

Dallabernardina, P., Ruprecht, C., Smith, P. J., Hahn, M. G., Urbanowicz, B. R., & Pfrengle, F. (2017). Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. Organic & Biomolecular Chemistry, 15(47), 9996-10000. doi:10.1039/C7OB02605F.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-7C4B-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-3573-3
Genre: Journal Article

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 Creators:
Dallabernardina, Pietro1, Author              
Ruprecht, Colin1, Author              
Smith, Peter J., Author
Hahn, Michael G., Author
Urbanowicz, Breeanna R., Author
Pfrengle, Fabian1, Author              
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Open Access
 Abstract: We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

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 Dates: 2017-11-212017-12-21
 Publication Status: Published in print
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 Identifiers: DOI: 10.1039/C7OB02605F
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Funding program : Emmy Noether Program (PF850/1-1)
Funding organization : German Research Foundation (DFG)

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Title: Organic & Biomolecular Chemistry
  Other : Organic and Biomolecular Chemistry
  Abbreviation : Org. Biomol. Chem.
Source Genre: Journal
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Publ. Info: Cambridge : Royal Society of Chemistry
Pages: - Volume / Issue: 15 (47) Sequence Number: - Start / End Page: 9996 - 10000 Identifier: ISSN: 1477-0520