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  Simultaneous determination of fast and slow dynamics in molecules using extreme CPMG relaxation dispersion experiments.

Reddy, J. G., Pratihar, S., Ban, D., Frischkorn, S., Becker, S., & Griesinger, C. (2018). Simultaneous determination of fast and slow dynamics in molecules using extreme CPMG relaxation dispersion experiments. Journal of Biomolecular NMR, 70(1), 1-9. doi:10.1007/s10858-017-0155-0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8135-D Version Permalink: http://hdl.handle.net/21.11116/0000-0003-AF97-E
Genre: Journal Article

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 Creators:
Reddy, J. G., Author
Pratihar, S.1, Author              
Ban, D.1, Author              
Frischkorn, S., Author
Becker, S.1, Author              
Griesinger, C.1, Author              
Affiliations:
1Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_578567              

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Free keywords: Protein dynamics; Relaxation dispersion; Extreme CPMG; Conformational exchange; μs–ms dynamics
 Abstract: Molecular dynamics play a significant role in how molecules perform their function. A critical method that provides information on dynamics, at the atomic level, is NMR-based relaxation dispersion (RD) experiments. RD experiments have been utilized for understanding multiple biological processes occurring at micro-to-millisecond time, such as enzyme catalysis, molecular recognition, ligand binding and protein folding. Here, we applied the recently developed high-power RD concept to the Carr–Purcell–Meiboom–Gill sequence (extreme CPMG; E-CPMG) for the simultaneous detection of fast and slow dynamics. Using a fast folding protein, gpW, we have shown that previously inaccessible kinetics can be accessed with the improved precision and efficiency of the measurement by using this experiment.

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Language(s): eng - English
 Dates: 2017-11-292018-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1007/s10858-017-0155-0
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 70 (1) Sequence Number: - Start / End Page: 1 - 9 Identifier: -