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  Anaerobically grown Escherichia coli has an enhanced mutation rate and distinct mutational spectra

Shewaramani, S., Finn, T. J., Leahy, S. C., Kassen, R., Rainey, P. B., & Moon, C. D. (2017). Anaerobically grown Escherichia coli has an enhanced mutation rate and distinct mutational spectra. PLoS Genetics, 13(1): e1006570. doi:10.1371/journal.pgen.1006570.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8218-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8219-5
Genre: Journal Article

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 Creators:
Shewaramani, Sonal, Author
Finn, Thomas J., Author
Leahy, Sinead C., Author
Kassen, Rees, Author
Rainey, Paul B.1, Author              
Moon, Christina D., Author
Affiliations:
1Department Microbial Population Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2421699              

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 Abstract: Author Summary When organisms are exposed to different environments, the rates and types of mutations that spontaneously arise in each environment can vary due to differing mutagenic pressures imposed by each environment, and these can potentially influence the evolution of the organism. Little is known about the types of mutations that arise when facultative anaerobes are grown in the absence of oxygen. To investigate the effect of oxygen availability on spontaneous mutation at the genome level, we directly measured the mutation rates of bacteria that were grown with and without oxygen under controlled laboratory conditions. To our knowledge, we have obtained the most comprehensive mutation rate estimate of anaerobically grown Escherichia coli, where mutation rates are higher in the absence of oxygen. We found that genomic structural variations, a class of mutations that are typically not investigated due to technical difficulties, were more prevalent under anaerobic growth conditions. We also identified distinct DNA strand biases for substitution types under aerobic and anaerobic growth conditions. Our findings provide new insights into the impact that oxygen availability may have on spontaneous mutations, and the mutational processes that underlie them.

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Language(s): eng - English
 Dates: 2016-06-022017-01-042017-01-192017-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1371/journal.pgen.1006570
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Funding program : Royal Society of New Zealand Marsden Fund (AGR0902)
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Title: PLoS Genetics
  Other : PLoS Genet.
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: 22 Volume / Issue: 13 (1) Sequence Number: e1006570 Start / End Page: - Identifier: ISSN: 1553-7390
CoNE: /journals/resource/1000000000017180