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  Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading

Böttcher, R. T., Veelders, M., Rombaut, P., Faix, J., Theodosiou, M., Stradal, T. E., et al. (2017). Kindlin-2 recruits paxillin and Arp2/3 to promote membrane protrusions during initial cell spreading. Journal of Cell Biology, 216(11), 3785-3798. doi:10.1083/jcb.201701176.

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© 2017 Böttcher et al.

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 Creators:
Böttcher, Ralph T.1, Author              
Veelders, Maik1, Author              
Rombaut, Pascaline2, Author
Faix, Jan2, Author
Theodosiou, Marina1, Author              
Stradal, Theresa E.2, Author
Rottner, Klemens2, Author
Zent, Roy2, Author
Herzog, Franz2, Author
Fässler, Reinhard1, Author              
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
2external, ou_persistent22              

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Free keywords: CHEMICAL CROSS-LINKING; FOCAL ADHESION KINASE; MASS-SPECTROMETRY; INTEGRIN ALPHA-IIB-BETA-3; PLATELET-AGGREGATION; ACTIN POLYMERIZATION; N-WASP; COMPLEX; PROTEIN; ACTIVATIONCell Biology;
 Abstract: Cell spreading requires the coupling of actin-driven membrane protrusion and integrin-mediated adhesion to the extracellular matrix. The integrin-activating adaptor protein kindlin-2 plays a central role for cell adhesion and membrane protrusion by directly binding and recruiting paxillin to nascent adhesions. Here, we report that kindlin-2 has a dual role during initial cell spreading: it binds paxillin via the pleckstrin homology and F0 domains to activate Rac1, and it directly associates with the Arp2/3 complex to induce Rac1-mediated membrane protrusions. Consistently, abrogation of kindlin-2 binding to Arp2/3 impairs lamellipodia formation and cell spreading. Our findings identify kindlin-2 as a key protein that couples cell adhesion by activating integrins and the induction of membrane protrusions by activating Rac1 and supplying Rac1 with the Arp2/3 complex.

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Language(s): eng - English
 Dates: 2017-09-142017
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000414609700028
DOI: 10.1083/jcb.201701176
 Degree: -

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Project name : Deutsche Forschungsgemeinschaft (SFB 914, project A05)
Grant ID : 322652
Funding program : -
Funding organization : European Research Council

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Title: Journal of Cell Biology
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 216 (11) Sequence Number: - Start / End Page: 3785 - 3798 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024_1