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  Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine

Warnke, E., Pietsch, J., Kopp, S., Bauer, J., Sahana, J., Wehland, M., et al. (2017). Cytokine Release and Focal Adhesion Proteins in Normal Thyroid Cells Cultured on the Random Positioning Machine. Cellular Physiology and Biochemistry, 43(1), 257-270. doi:10.1159/000480368.

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© 2017 The Author(s). This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND).
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 Creators:
Warnke, Elisabeth1, Author
Pietsch, Jessica1, Author
Kopp, Sascha1, Author
Bauer, Johann2, Author              
Sahana, Jayashree1, Author
Wehland, Markus1, Author
Krueger, Marcus1, Author
Hemmersbach, Ruth1, Author
Infanger, Manfred1, Author
Luetzenberg, Ronald1, Author
Grimm, Daniela1, Author
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1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: ENDOTHELIAL GROWTH-FACTOR; MONOCYTE CHEMOATTRACTANT PROTEIN-1; SIMULATED MICROGRAVITY CONDITIONS; CANCER CELLS; SPHEROID FORMATION; EXTRACELLULAR-MATRIX; 3-DIMENSIONAL GROWTH; UP-REGULATION; CARCINOMA-CELLS; MECHANISMSCell Biology; Physiology; Thyroid; Multicellular spheroids; Cytokines; Focal adhesion; Extracellular matrix proteins; Pathway analyses;
 Abstract: Background/Aims: Spaceflight impacts on the function of the thyroid gland in vivo. In vitro normal and malignant thyrocytes assemble in part to multicellular spheroids (MCS) after exposure to the random positioning machine (RPM), while a number of cells remain adherent (AD). We aim to elucidate possible differences between AD and MCS cells compared to 1g-controls of normal human thyroid cells. Methods: Cells of the human follicular epithelial thyroid cell line Nthy-ori 3-1 were incubated for up to 72 h on the RPM. Afterwards, they were investigated by phase-contrast microscopy, quantitative real-time PCR and by determination of cytokines released in their supernatants. Results: A significant up-regulation of IL6, IL8 and CCL2 gene expression was found after a 4h RPM-exposure, when the whole population was still growing adherently. MCS and AD cells were detected after 24 h on the RPM. At this time, a significantly reduced gene expression in MCS compared to 1g-controls was visible for IL6, IL8, FN1, ITGB1, LAMA1, CCL2, and TLN1. After a 72 h RPM-exposure, IL-6, IL-8, and TIMP-1 secretion rates were increased significantly. Conclusion: Normal thyrocytes form MCS within 24 h. Cytokines seem to be involved in the initiation of MCS formation via focal adhesion proteins. (C) 2017 The Author(s) Published by S. Karger AG, Basel

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Language(s): eng - English
 Dates: 2017
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000415240500021
DOI: 10.1159/000480368
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Title: Cellular Physiology and Biochemistry
Source Genre: Journal
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Publ. Info: Basel : S. Karger
Pages: - Volume / Issue: 43 (1) Sequence Number: - Start / End Page: 257 - 270 Identifier: ISSN: 1015-8987
CoNE: https://pure.mpg.de/cone/journals/resource/954925585261