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  Visually evoked activity in cortical cells imaged in freely moving animals

Sawinski, J., Haydon-Wallace, D. J., Greenberg, D. S., Grossmann, S., Denk, W., & Kerr, J. (2009). Visually evoked activity in cortical cells imaged in freely moving animals. Proceedings of the National Academy of Sciences of the United States of America, 106(46), 19557-19562. doi:10.1073/pnas.0903680106.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-85F3-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-85F4-1
Genre: Journal Article
Alternative Title : Visually evoked activity in cortical cells imaged in freely moving animals

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PNAS_106_2009_19557.pdf (Any fulltext), 3MB
 
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 Creators:
Sawinski, Jürgen1, Author              
Haydon-Wallace, Damian J.2, Author              
Greenberg, D. S., Author
Grossmann, Silvie, Author
Denk, Winfried1, Author              
Kerr, Jason1, 2, Author              
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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Free keywords: calcium imaging, head-mounted microscope neuronal activity, two-photon, visual cortex
 Abstract: We describe a miniaturized head-mounted multiphoton microscope and its use for recording Ca2+ transients from the somata of layer 2/3 neurons in the visual cortex of awake, freely moving rats. Images contained up to 20 neurons and were stable enough to record continuously for >5 min per trial and 20 trials per imaging session, even as the animal was running at velocities of up to 0.6 m/s. Neuronal Ca2+ transients were readily detected, and responses to various static visual stimuli were observed during free movement on a running track. Neuronal activity was sparse and increased when the animal swept its gaze across a visual stimulus. Neurons showing preferential activation by specific stimuli were observed in freely moving animals. These results demonstrate that the multiphoton fiberscope is suitable for functional imaging in awake and freely moving animals.

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Language(s): eng - English
 Dates: 2009-04-042009-11-042009-11-17
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: National Academy of Sciences
Pages: - Volume / Issue: 106 (46) Sequence Number: - Start / End Page: 19557 - 19562 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230