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  Copy number increases of transposable elements and protein-coding genes in an invasive fish of hybrid origin

Dennenmoser, S., Sedlazeck, F. J., Iwaszkiewicz, E., Li, X.-Y., Altmüller, J., & Nolte, A. W. (2017). Copy number increases of transposable elements and protein-coding genes in an invasive fish of hybrid origin. Molecular Ecology, 26(18), 4712-4724. doi:10.1111/mec.14134.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-89A4-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-89A5-4
Genre: Journal Article

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https://creativecommons.org/licenses/by/4.0/

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 Creators:
Dennenmoser, Stefan1, Author              
Sedlazeck, Fritz J., Author
Iwaszkiewicz, Elzbieta1, Author              
Li, Xiang-Yi, Author
Altmüller, Janine, Author
Nolte, Arne W.1, Author              
Affiliations:
1Research Group Evolutionary Genetics of Fishes, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445645              

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Free keywords: array-comparative genomic hybridization; Cottidae; digital droplet PCR; hybrid speciation; invasion genetics; structural mutations
 Abstract: Evolutionary dynamics of structural genetic variation in lineages of hybrid origin is not well explored, although structural mutations may increase in controlled hybrid crosses. We therefore tested whether structural variants accumulate in a fish of recent hybrid origin, invasive Cottus, relative to both parental species Cottus rhenanus and Cottus perifretum. Copy-number variation in exons of 10,979 genes was assessed using comparative genome hybridization arrays. Twelve genes showed significantly higher copy numbers in invasive Cottus compared to both parents. This coincided with increased expression for three genes related to vision, detoxification and muscle development, suggesting possible gene dosage effects. Copy number increases of putative transposons were assessed by comparative mapping of genomic DNA reads against a de novo assembly of 1,005 repetitive elements. In contrast to exons, copy number increases of repetitive elements were common (20.7) in invasive Cottus, whereas decrease was very rare (0.01). Among the increased repetitive elements, 53.8 occurred at higher numbers in C. perifretum compared to C. rhenanus, while only 1.4 were more abundant in C. rhenanus. This implies a biased mutational process that amplifies genetic material from one ancestor. To assess the frequency of de novo mutations through hybridization, we screened 64 laboratory-bred F2 offspring between the parental species for copy-number changes at five candidate loci. We found no evidence for new structural variants, indicating that they are too rare to be detected given our sampling scheme. Instead, they must have accumulated over more generations than we observed in a controlled cross.

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Language(s): eng - English
 Dates: 2017-03-232017-01-042017-03-272017
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1111/mec.14134
 Degree: -

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Project name : H2020 European Research Council
Grant ID : IOS-1237880
Funding program : National Science Foundation (DBI-1350041)
Funding organization : -

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Title: Molecular Ecology
Source Genre: Journal
 Creator(s):
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 26 (18) Sequence Number: - Start / End Page: 4712 - 4724 Identifier: ISSN: 0962-1083
CoNE: /journals/resource/954925580119