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  Structure of the recombinant antibody Fab fragment f3p4

Frey, D., Huber, T., Plückthun, A., & Grütter, M. G. (2008). Structure of the recombinant antibody Fab fragment f3p4. Acta Crystallographica Section D-Biological Crystallography, 64(6), 636-643. doi:10.1107/S0907444908007282.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8DCE-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-E3DE-6
Genre: Journal Article

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ActaCrystD_64_2008_636.pdf (Any fulltext), 2MB
 
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 Creators:
Frey, Daniel1, Author              
Huber, Thomas, Author
Plückthun, Andreas, Author
Grütter, Markus G., Author
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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Free keywords: recombinant antibody libraries; Fab fragments; pseudomerohedral twinning; cocrystallization of membrane proteins
 Abstract: The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.

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Language(s): eng - English
 Dates: 2008-01-142008-03-172008-05-142008-06
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Acta Crystallographica Section D-Biological Crystallography
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 64 (6) Sequence Number: - Start / End Page: 636 - 643 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619_1