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  Magneto-active substrates for local mechanical stimulation of living cells

Bidan, C. M., Fratzl, M., Coullomb, A., Moreau, P., Lombard, A. H., Wang, I., et al. (2018). Magneto-active substrates for local mechanical stimulation of living cells. Scientific Reports, 1464. doi:10.1038/s41598-018-19804-1.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-8F8E-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0000-341F-4
Genre: Journal Article

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Bidan, Cécile M.1, Author              
Fratzl, Mario, Author
Coullomb, Alexis, Author
Moreau, Philippe, Author
Lombard, Alain H, Author
Wang, Irène, Author
Balland, Martial, Author
Boudou, Thomas, Author
Dempsey, Nora M, Author
Devillers, Thibaut, Author
Dupont, Aurélie, Author
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1External Organizations, ou_persistent22              

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 Abstract: Cells are able to sense and react to their physical environment by translating a mechanical cue into an intracellular biochemical signal that triggers biological and mechanical responses. This process, called mechanotransduction, controls essential cellular functions such as proliferation and migration. The cellular response to an external mechanical stimulation has been investigated with various static and dynamic systems, so far limited to global deformations or to local stimulation through discrete substrates. To apply local and dynamic mechanical constraints at the single cell scale through a continuous surface, we have developed and modelled magneto-active substrates made of magnetic micro-pillars embedded in an elastomer. Constrained and unconstrained substrates are analysed to map surface stress resulting from the magnetic actuation of the micro-pillars and the adherent cells. These substrates have a rigidity in the range of cell matrices, and the magnetic micro-pillars generate local forces in the range of cellular forces, both in traction and compression. As an application, we followed the protrusive activity of cells subjected to dynamic stimulations. Our magneto-active substrates thus represent a new tool to study mechanotransduction in single cells, and complement existing techniques by exerting a local and dynamic stimulation, traction and compression, through a continuous soft substrate.

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 Dates: 2018
 Publication Status: Published in print
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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: - Sequence Number: 1464 Start / End Page: - Identifier: ISSN: 2045-2322